| Literature DB >> 21762807 |
Nancy L Goicochea1, Siddhartha A K Datta, Murali Ayaluru, Cheng Kao, Alan Rein, Bogdan Dragnea.
Abstract
Size polydispersity of immature human immunodeficiency virus type 1 (HIV-1) particles represents a challenge for traditional methods of biological ultrastructural analysis. An in vitro model for immature HIV-1 particles constructed from recombinant Gag proteins lacking residues 16-99 and the p6 domain assembled around spherical nanoparticles functionalized with DNA. This template-directed assembly approach led to a significant reduction in size polydispersity and revealed previously unknown structural features of immature-like HIV-1 particles. Electron microscopy and image reconstruction of these particles suggest that the Gag shell formed from different protein regions that are connected by a "scar"-an extended defect connecting the edges of two continuous, regularly packed protein layers. Thus, instead of a holey protein array, the experimental model presented here appears to consist of a continuous array of ∼5000 proteins enveloping the core, in which regular regions are separated by extended areas of disorder.Entities:
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Year: 2011 PMID： 21762807 PMCID： PMC3140650 DOI： 10.1016/j.jmb.2011.04.012
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469