Literature DB >> 21761290

Laser capture microdissection: methods and applications.

Kristen Decarlo1, Andrew Emley, Ophelia E Dadzie, Meera Mahalingam.   

Abstract

Laser microdissection is a nonmolecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. It is a versatile technology and allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.

Mesh:

Substances:

Year:  2011        PMID: 21761290     DOI: 10.1007/978-1-61779-163-5_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  24 in total

Review 1.  Approaches for targeted proteomics and its potential applications in neuroscience.

Authors:  Sumit Sethi; Dipti Chourasia; Ishwar S Parhar
Journal:  J Biosci       Date:  2015-09       Impact factor: 1.826

Review 2.  Dual RNA-seq of pathogen and host.

Authors:  Alexander J Westermann; Stanislaw A Gorski; Jörg Vogel
Journal:  Nat Rev Microbiol       Date:  2012-09       Impact factor: 60.633

3.  RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling.

Authors:  Audrey Chabrat; Hélène Doucet-Beaupré; Martin Lévesque
Journal:  J Vis Exp       Date:  2015-04-11       Impact factor: 1.355

4.  Enrichment of single neurons and defined brain regions from human brain tissue samples for subsequent proteome analysis.

Authors:  Mariana Molina; Simone Steinbach; Young Mok Park; Su Yeong Yun; Ana Tereza Di Lorenzo Alho; Helmut Heinsen; Lea T Grinberg; Katrin Marcus; Renata E Paraizo Leite; Caroline May
Journal:  J Neural Transm (Vienna)       Date:  2015-06-30       Impact factor: 3.575

5.  Laser capture microdissection protocol for gene expression analysis in the brain.

Authors:  P Garrido-Gil; P Fernandez-Rodríguez; J Rodríguez-Pallares; Jose L Labandeira-Garcia
Journal:  Histochem Cell Biol       Date:  2017-05-31       Impact factor: 4.304

6.  Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection.

Authors:  Hitokazu Esaki; David A Ewald; Benjamin Ungar; Mariya Rozenblit; Xiuzhong Zheng; Hui Xu; Yeriel D Estrada; Xiangyu Peng; Hiroshi Mitsui; Thomas Litman; Mayte Suárez-Fariñas; James G Krueger; Emma Guttman-Yassky
Journal:  J Allergy Clin Immunol       Date:  2015-01       Impact factor: 10.793

7.  Magnetic Cell Sorting for In Vivo and In Vitro Astrocyte, Neuron, and Microglia Analysis.

Authors:  Leanne M Holt; S Tristan Stoyanof; Michelle L Olsen
Journal:  Curr Protoc Neurosci       Date:  2019-06

8.  Epigenetic analysis of laser capture microdissected fetal epithelia.

Authors:  Ratnam S Seelan; Dennis R Warner; Partha M Mukhopadhyay; Sarah A Andres; Irina A Smolenkova; James L Wittliff; M Michele Pisano; Robert M Greene
Journal:  Anal Biochem       Date:  2013-07-30       Impact factor: 3.365

9.  Oligonucleotide modifications enhance probe stability for single cell transcriptome in vivo analysis (TIVA).

Authors:  S B Yeldell; B K Ruble; I J Dmochowski
Journal:  Org Biomol Chem       Date:  2017-12-06       Impact factor: 3.876

Review 10.  Technologies for systems-level analysis of specific cell types in plants.

Authors:  Dongxue Wang; E Shannon Mills; Roger B Deal
Journal:  Plant Sci       Date:  2012-08-30       Impact factor: 4.729

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.