Literature DB >> 21748690

Fluorescence recovery after photobleaching.

Alex Carisey1, Matthew Stroud, Ricky Tsang, Christoph Ballestrem.   

Abstract

This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.

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Year:  2011        PMID： 21748690     DOI： 10.1007/978-1-61779-207-6_26

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Herpesviruses induce aggregation and selective autophagy of host signalling proteins NEMO and RIPK1 as an immune-evasion mechanism.

Authors:  Elena Muscolino; Rebekka Schmitz; Stefan Loroch; Enrico Caragliano; Carola Schneider; Matteo Rizzato; Young-Hyun Kim; Eva Krause; Vanda Juranić Lisnić; Albert Sickmann; Rudolph Reimer; Eleonore Ostermann; Wolfram Brune
Journal:  Nat Microbiol       Date:  2019-12-16       Impact factor: 17.745

Review 2.  FRAP, FLIM, and FRET: Detection and analysis of cellular dynamics on a molecular scale using fluorescence microscopy.

Authors:  Carla De Los Santos; Ching-Wei Chang; Mary-Ann Mycek; Richard A Cardullo
Journal:  Mol Reprod Dev       Date:  2015-05-25       Impact factor: 2.609

3.  Distinct focal adhesion protein modules control different aspects of mechanotransduction.

Authors:  Ben Stutchbury; Paul Atherton; Ricky Tsang; De-Yao Wang; Christoph Ballestrem
Journal:  J Cell Sci       Date:  2017-03-16       Impact factor: 5.285

4.  Desmosome dualism - most of the junction is stable, but a plakophilin moiety is persistently dynamic.

Authors:  Judith B Fülle; Henri Huppert; David Liebl; Jaron Liu; Rogerio Alves de Almeida; Bian Yanes; Graham D Wright; E Birgitte Lane; David R Garrod; Christoph Ballestrem
Journal:  J Cell Sci       Date:  2021-11-10       Impact factor: 5.285

5.  Src kinase determines the dynamic exchange of the docking protein NEDD9 (neural precursor cell expressed developmentally down-regulated gene 9) at focal adhesions.

Authors:  Peta Bradbury; Cuc T Bach; Andre Paul; Geraldine M O'Neill
Journal:  J Biol Chem       Date:  2014-07-24       Impact factor: 5.157

6.  Live-cell confocal microscopy and quantitative 4D image analysis of anchor-cell invasion through the basement membrane in Caenorhabditis elegans.

Authors:  Laura C Kelley; Zheng Wang; Elliott J Hagedorn; Lin Wang; Wanqing Shen; Shijun Lei; Sam A Johnson; David R Sherwood
Journal:  Nat Protoc       Date:  2017-09-07       Impact factor: 13.491

7.  Characterization of G2L3 (GAS2-like 3), a new microtubule- and actin-binding protein related to spectraplakins.

Authors:  Matthew J Stroud; Richard A Kammerer; Christoph Ballestrem
Journal:  J Biol Chem       Date:  2011-05-11       Impact factor: 5.157

8.  H3K9 methylation extends across natural boundaries of heterochromatin in the absence of an HP1 protein.

Authors:  Rieka Stunnenberg; Raghavendran Kulasegaran-Shylini; Claudia Keller; Moritz A Kirschmann; Laurent Gelman; Marc Bühler
Journal:  EMBO J       Date:  2015-10-05       Impact factor: 11.598

9.  Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules.

Authors:  Sonja Kroschwald; Shovamayee Maharana; Daniel Mateju; Liliana Malinovska; Elisabeth Nüske; Ina Poser; Doris Richter; Simon Alberti
Journal:  Elife       Date:  2015-08-04       Impact factor: 8.140

10.  GAS2-like proteins mediate communication between microtubules and actin through interactions with end-binding proteins.

Authors:  Matthew J Stroud; Alicja Nazgiewicz; Edward A McKenzie; Yisu Wang; Richard A Kammerer; Christoph Ballestrem
Journal:  J Cell Sci       Date:  2014-04-04       Impact factor: 5.285
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