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Literature DB >> 21741952

Development of a universal glycosyltransferase assay amenable to high-throughput formats.

Abstract

The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.

Entities: Chemical Disease Gene Species

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Year: 2011 PMID： 21741952 PMCID： PMC3354722 DOI： 10.1016/j.ab.2011.06.016

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

24 in total

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Authors:
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9. Universal phosphatase-coupled glycosyltransferase assay.

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Review 10. Synthesis and medical applications of oligosaccharides.

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7 in total

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Authors: Shilpa A Patil; E V Chandrasekaran; Khushi L Matta; Abhirath Parikh; Emmanuel S Tzanakakis; Sriram Neelamegham
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