Literature DB >> 217356

Preparative-scale enzymic synthesis of D-[14C]ribulose 1,5-bisphosphate.

G D Kuehn, T C Hsu.   

Abstract

A procedure is described to prepare uniformly labelled D-[14C]ribulose 1,5-bisphosphate enzymically from uniformly labelled D-[14C]glucose through the coupled reactions catalysed by hexokinase (EC 2.7.1.1), glucose 6-phosphate dehydrogenase (EC 1.1.1.49), 6-phosphogluconate dehydrogenase (EC 1.1.1.44) and 5-phosphoribulokinase (EC 2.7.1.19). All reagents utilized in the method are commercially available. The procedure is a reliable preparative-scale method for synthesizing the dibarium salt of D-[14C]ribulose 1,5-biphosphate with a specific radioactivity up to 7 mCi/mmol and a purity near 90%. The final product was free of other 14C-labelled sugars, sugar phosphate esters, Pi and nucleotides.

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Year:  1978        PMID： 217356      PMCID： PMC1186152          DOI： 10.1042/bj1750909

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  An improved solvent system for the paper chromatography of phosphate esters.

Authors:  E TYSZKIEWICZ
Journal:  Anal Biochem       Date:  1962-02       Impact factor: 3.365

2.  Separation of the phosphoric esters on the filter paper chromatogram.

Authors:  C S HANES; F A ISHERWOOD
Journal:  Nature       Date:  1949-12-31       Impact factor: 49.962

Review 3.  Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase.

Authors:  B A McFadden
Journal:  Bacteriol Rev       Date:  1973-09

4.  Ribulose diphosphate oxygenase. I. Synthesis of phosphoglycolate by fraction-1 protein of leaves.

Authors:  T J Andrews; G H Lorimer; N E Tolbert
Journal:  Biochemistry       Date:  1973-01-02       Impact factor: 3.162

5.  Ribulose 1,5-diphosphate carboxylase from Hydrogenomonas eutropha and Hydrogenomonas facilis. I. Purification, metallic ion requirements, inhibition, and kinetic constants.

Authors:  G D Kuehn; B A McFadden
Journal:  Biochemistry       Date:  1969-06       Impact factor: 3.162

6.  Inhibition of ribulose diphosphate carboxylase by cyanide. Inactive ternary complex of enzyme, ribulose diphosphate, and cyanide.

Authors:  M Wishnick; M D Lane
Journal:  J Biol Chem       Date:  1969-01-10       Impact factor: 5.157

7.  Paracatalytic enzyme modification by oxidation of enzyme-substrate carbanion intermediates.

Authors:  P Christen
Journal:  Methods Enzymol       Date:  1977       Impact factor: 1.600

8.  Uridine diphosphoglucose pyrophosphorylase activity and differentiation in the cellular slime mold Physarum polycephaluno.

Authors:  G D Kuehn
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490
  8 in total
  13 in total

1.  Substitutions at the opening of the Rubisco central solvent channel affect holoenzyme stability and CO2/O 2 specificity but not activation by Rubisco activase.

Authors:  M Gloria Esquivel; Todor Genkov; Ana S Nogueira; Michael E Salvucci; Robert J Spreitzer
Journal:  Photosynth Res       Date:  2013-09-07       Impact factor: 3.573

2.  Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis Is Not Due to Decarbamylation of the Catalytic Site.

Authors:  D L Edmondson; M R Badger; T J Andrews
Journal:  Plant Physiol       Date:  1990-08       Impact factor: 8.340

3.  Functional hybrid rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in chlamydomonas.

Authors:  Todor Genkov; Moritz Meyer; Howard Griffiths; Robert J Spreitzer
Journal:  J Biol Chem       Date:  2010-04-27       Impact factor: 5.157

4.  Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco.

Authors:  Robert J Spreitzer; Srinivasa R Peddi; Sriram Satagopan
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-10       Impact factor: 11.205

5.  Multiple catalytic roles of His 287 of Rhodospirillum rubrum ribulose 1,5-bisphosphate carboxylase/oxygenase.

Authors:  M R Harpel; F W Larimer; F C Hartman
Journal:  Protein Sci       Date:  1998-03       Impact factor: 6.725

6.  RbcS suppressor mutations improve the thermal stability and CO2/O2 specificity of rbcL- mutant ribulose-1,5-bisphosphate carboxylase/oxygenase.

Authors:  Y C Du; S Hong; R J Spreitzer
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

7.  A Sensitive Assay Procedure for Simultaneous Determination of Ribulose-1,5-bisphosphate Carboxylase and Oxygenase Activities.

Authors:  D B Jordan; W L Ogren
Journal:  Plant Physiol       Date:  1981-02       Impact factor: 8.340

8.  A Rubisco mutant that confers growth under a normally "inhibitory" oxygen concentration.

Authors:  Sriram Satagopan; Stephanie S Scott; Todd G Smith; F Robert Tabita
Journal:  Biochemistry       Date:  2009-09-29       Impact factor: 3.162

9.  Highly conserved small subunit residues influence rubisco large subunit catalysis.

Authors:  Todor Genkov; Robert J Spreitzer
Journal:  J Biol Chem       Date:  2009-09-04       Impact factor: 5.157

10.  Structure-function studies with the unique hexameric form II ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Rhodopseudomonas palustris.

Authors:  Sriram Satagopan; Sum Chan; L Jeanne Perry; F Robert Tabita
Journal:  J Biol Chem       Date:  2014-06-18       Impact factor: 5.157
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