Literature DB >> 21705538

Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.

Akihiro Ohnishi1, Shinko Abe, Shiho Nashirozawa, Sayaka Shimada, Naoshi Fujimoto, Masaharu Suzuki.   

Abstract

The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.

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Year:  2011        PMID： 21705538      PMCID： PMC3147432          DOI： 10.1128/AEM.00359-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Substrate Preference in a Strain of Megasphaera elsdenii, a Ruminal Bacterium, and Its Implications in Propionate Production and Growth Competition.

Authors:  T Hino; K Shimada; T Maruyama
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

2.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.

Authors:  J D Thompson; T J Gibson; F Plewniak; F Jeanmougin; D G Higgins
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971

Review 3.  Hydrogen: the future energy carrier.

Authors:  Andreas Züttel; Arndt Remhof; Andreas Borgschulte; Oliver Friedrichs
Journal:  Philos Trans A Math Phys Eng Sci       Date:  2010-07-28       Impact factor: 4.226

4.  Phylogenetic analysis of some Sporomusa sub-branch members isolated from human clinical specimens: description of Megasphaera micronuciformis sp. nov.

Authors:  Hélène Marchandin; Estelle Jumas-Bilak; Bernard Gay; Corinne Teyssier; Hélène Jean-Pierre; Michèle Siméon de Buochberg; Christian Carrière; Jean-Philippe Carlier
Journal:  Int J Syst Evol Microbiol       Date:  2003-03       Impact factor: 2.747

5.  Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus.

Authors:  Riikka Juvonen; Maija-Liisa Suihko
Journal:  Int J Syst Evol Microbiol       Date:  2006-04       Impact factor: 2.747

6.  Megasphaera elsdenii JCM1772T normalizes hyperlactate production in the large intestine of fructooligosaccharide-fed rats by stimulating butyrate production.

Authors:  Kenta Hashizume; Takamitsu Tsukahara; Kouji Yamada; Hironari Koyama; Kazunari Ushida
Journal:  J Nutr       Date:  2003-10       Impact factor: 4.798

7.  Presence of lactate dehydrogenase and lactate racemase in Megasphaera elsdenii grown on glucose or lactate.

Authors:  T Hino; S Kuroda
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792
  7 in total
  3 in total

1.  Diet-driven microbial ecology underpins associations between cancer immunotherapy outcomes and the gut microbiome.

Authors:  Rebecca C Simpson; Erin R Shanahan; Marcel Batten; Irene L M Reijers; Mark Read; Ines P Silva; Judith M Versluis; Rosilene Ribeiro; Alexandra S Angelatos; Jian Tan; Chandra Adhikari; Alexander M Menzies; Robyn P M Saw; Maria Gonzalez; Kerwin F Shannon; Andrew J Spillane; Rebecca Velickovic; Alexander J Lazar; Ashish V Damania; Aditya K Mishra; Manoj Chelvanambi; Anik Banerjee; Nadim J Ajami; Jennifer A Wargo; Laurence Macia; Andrew J Holmes; James S Wilmott; Christian U Blank; Richard A Scolyer; Georgina V Long
Journal:  Nat Med       Date:  2022-09-22       Impact factor: 87.241

2.  Differential desulfurization of dibenzothiophene by newly identified MTCC strains: Influence of Operon Array.

Authors:  Madhabi M Bhanjadeo; Kalyani Rath; Dhirendra Gupta; Nilotpala Pradhan; Surendra K Biswal; Barada K Mishra; Umakanta Subudhi
Journal:  PLoS One       Date:  2018-03-08       Impact factor: 3.240

3.  Veillonellaceae family members uniquely alter the cervical metabolic microenvironment in a human three-dimensional epithelial model.

Authors:  Mary E Salliss; Jason D Maarsingh; Camryn Garza; Paweł Łaniewski; Melissa M Herbst-Kralovetz
Journal:  NPJ Biofilms Microbiomes       Date:  2021-07-06       Impact factor: 7.290
  3 in total

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