BACKGROUND: Small GTPase proteins, including RhoA, RhoB, RhoC, Rac1, and cdc42, are important molecules for linking cell shape and cell-cycle progression because of their role in both cytoskeletal arrangements and mitogenic signaling. Over-expression of wild-type or constitutively active forms of RhoA has been shown to induce invasive behavior in non-invasive rat hepatoma cells in vitro. In addition, over-expression of RhoC has been found in melanoma cells with increasing metastatic activity as well as inflammatory breast cancer. These results indicate that overexpression of Rho proteins contributes to cancer cell invasion and metastasis. Rho GDP dissociation inhibitor 2 (RhoGDI2) was recently shown to act as a metastasis suppressor gene in bladder cancer. The purpose of this study was to clarify the clinical significance of this gene expression in patients with colorectal carcinoma. METHODS: Fifty pairs of normal mucosa and cancer specimens obtained at the time of surgery from patients with colorectal cancer (CRC) were subjected to reverse transcription-polymerase chain reaction for RhoGDI2. RESULTS: No patients with RhoGDI2-higher expression tumors had liver metastasis (0 in 8 cases); however, 33.3% (14 in 42 cases) of patients with RhoGDI2-lower expression tumors had liver metastasis. With regard to outcome in relation to RhoGDI2-positivity, RhoGDI2-higher expression tumors had a significant correlation with superior relapse-free survival (RFS) time as compared to RhoGDI2-lower expression tumors in stage III CRC (log-rank test, P < 0.05). Moreover, multivariate analysis indicated that RhoGDI2 was an independent prognostic factor for RFS. CONCLUSION: RhoGDI2 is a novel predictor of RFS in patients with colorectal carcinoma.
BACKGROUND: Small GTPase proteins, including RhoA, RhoB, RhoC, Rac1, and cdc42, are important molecules for linking cell shape and cell-cycle progression because of their role in both cytoskeletal arrangements and mitogenic signaling. Over-expression of wild-type or constitutively active forms of RhoA has been shown to induce invasive behavior in non-invasive rathepatoma cells in vitro. In addition, over-expression of RhoC has been found in melanoma cells with increasing metastatic activity as well as inflammatory breast cancer. These results indicate that overexpression of Rho proteins contributes to cancer cell invasion and metastasis. Rho GDP dissociation inhibitor 2 (RhoGDI2) was recently shown to act as a metastasis suppressor gene in bladder cancer. The purpose of this study was to clarify the clinical significance of this gene expression in patients with colorectal carcinoma. METHODS: Fifty pairs of normal mucosa and cancer specimens obtained at the time of surgery from patients with colorectal cancer (CRC) were subjected to reverse transcription-polymerase chain reaction for RhoGDI2. RESULTS: No patients with RhoGDI2-higher expression tumors had liver metastasis (0 in 8 cases); however, 33.3% (14 in 42 cases) of patients with RhoGDI2-lower expression tumors had liver metastasis. With regard to outcome in relation to RhoGDI2-positivity, RhoGDI2-higher expression tumors had a significant correlation with superior relapse-free survival (RFS) time as compared to RhoGDI2-lower expression tumors in stage III CRC (log-rank test, P < 0.05). Moreover, multivariate analysis indicated that RhoGDI2 was an independent prognostic factor for RFS. CONCLUSION:RhoGDI2 is a novel predictor of RFS in patients with colorectal carcinoma.
Authors: Konstadinos Moissoglu; Kevin S McRoberts; Jeremy A Meier; Dan Theodorescu; Martin A Schwartz Journal: Cancer Res Date: 2009-03-10 Impact factor: 12.701
Authors: Dan Theodorescu; L M Sapinoso; M R Conaway; G Oxford; G M Hampton; H F Frierson Journal: Clin Cancer Res Date: 2004-06-01 Impact factor: 12.531
Authors: Yimin Wu; Konstadinos Moissoglu; Hong Wang; Xuejiao Wang; Henry F Frierson; Martin A Schwartz; Dan Theodorescu Journal: Proc Natl Acad Sci U S A Date: 2009-03-25 Impact factor: 11.205
Authors: Amos H P Loh; Clara Angelina; Meng Kang Wong; Sheng Hui Tan; Sarvesh A Sukhatme; Trifanny Yeo; Su Bin Lim; York Tien Lee; Shui Yen Soh; Wing Leung; Kenneth T E Chang; Yong Wei Chua; Syed M F Alkaff; Tony K H Lim; Chwee Teck Lim; Zhi Xiong Chen Journal: Front Oncol Date: 2022-09-13 Impact factor: 5.738