Literature DB >> 21697325

Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus and associated toxin genes.

G E Fosheim1, A C Nicholson, V S Albrecht, B M Limbago.   

Abstract

We describe a real-time PCR assay for the detection of methicillin-resistant Staphylococcus aureus and genes encoding toxic shock syndrome toxin 1 and Panton-Valentine leukocidin. Rapid screening and detection of toxins is a useful tool for surveillance studies and outbreak investigations involving large numbers of isolates.

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Year:  2011        PMID： 21697325      PMCID： PMC3147714          DOI： 10.1128/JCM.00795-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  A 5' nuclease PCR (TaqMan) high-throughput assay for detection of the mecA gene in staphylococci.

Authors:  G E Killgore; B Holloway; F C Tenover
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  A nuc-deficient methicillin-resistant Staphylococcus aureus strain.

Authors:  Willem van Leeuwen; Lieuwe Roorda; Wim Hendriks; Patrice Francois; Jacques Schrenzel
Journal:  FEMS Immunol Med Microbiol       Date:  2008-09-22

3.  Development of a real-time Staphylococcus aureus and MRSA (SAM-) PCR for routine blood culture.

Authors:  L C Thomas; H F Gidding; A N Ginn; T Olma; J Iredell
Journal:  J Microbiol Methods       Date:  2006-10-12       Impact factor: 2.363

4.  Detection of mecA-mediated resistance using reference and commercial testing methods in a collection of Staphylococcus aureus expressing borderline oxacillin MICs.

Authors:  Jana M Swenson; David Lonsway; Sigrid McAllister; Angela Thompson; Laura Jevitt; Wenming Zhu; Jean B Patel
Journal:  Diagn Microbiol Infect Dis       Date:  2007-01-19       Impact factor: 2.803

5.  A genetic system for analysis of staphylococcal nuclease.

Authors:  D Shortle
Journal:  Gene       Date:  1983 May-Jun       Impact factor: 3.688

6.  A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes.

Authors:  Ritu Banerjee; Michael Gretes; Christopher Harlem; Li Basuino; Henry F Chambers
Journal:  Antimicrob Agents Chemother       Date:  2010-08-30       Impact factor: 5.191

7.  Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

Authors:  K Ubukata; R Nonoguchi; M Matsuhashi; M Konno
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

8.  Characterization of methicillin-resistant Staphylococcus aureus isolates collected in 2005 and 2006 from patients with invasive disease: a population-based analysis.

Authors:  Brandi Limbago; Gregory E Fosheim; Valerie Schoonover; Christina E Crane; Joelle Nadle; Susan Petit; David Heltzel; Susan M Ray; Lee H Harrison; Ruth Lynfield; Ghinwa Dumyati; John M Townes; William Schaffner; Yi Mu; Scott K Fridkin
Journal:  J Clin Microbiol       Date:  2009-03-25       Impact factor: 5.948
  8 in total
  19 in total

1.  Molecular characterization of a catalase-negative methicillin-susceptible Staphylococcus aureus subsp. aureus strain collected from a patient with cutaneous abscess.

Authors:  Michael W Ellis; Ryan C Johnson; Katrina Crawford; Jeffrey B Lanier; D Scott Merrell
Journal:  J Clin Microbiol       Date:  2013-10-16       Impact factor: 5.948

2.  Hygiene strategies to prevent methicillin-resistant Staphylococcus aureus skin and soft tissue infections: a cluster-randomized controlled trial among high-risk military trainees.

Authors:  Michael W Ellis; Carey D Schlett; Eugene V Millar; Kenneth J Wilkins; Katrina B Crawford; Stephanie M Morrison-Rodriguez; Laura A Pacha; Rachel J Gorwitz; Jeffrey B Lanier; David R Tribble
Journal:  Clin Infect Dis       Date:  2014-03-14       Impact factor: 9.079

3.  Transmission of methicillin-resistant Staphylococcus aureus infection through solid organ transplantation: confirmation via whole genome sequencing.

Authors:  J M Wendt; D Kaul; B M Limbago; M Ramesh; S Cohle; A M Denison; E M Driebe; J K Rasheed; S R Zaki; D M Blau; C D Paddock; L K McDougal; D M Engelthaler; P S Keim; C C Roe; H Akselrod; M J Kuehnert; S V Basavaraju
Journal:  Am J Transplant       Date:  2014-09-22       Impact factor: 8.086

4.  Rapid detection of live methicillin-resistant Staphylococcus aureus by using an integrated microfluidic system capable of ethidium monoazide pre-treatment and molecular diagnosis.

Authors:  Yu-Hsin Liu; Chih-Hung Wang; Jiunn-Jong Wu; Gwo-Bin Lee
Journal:  Biomicrofluidics       Date:  2012-09-10       Impact factor: 2.800

5.  Recurrent Methicillin-Resistant Staphylococcus aureus Cutaneous Abscesses and Selection of Reduced Chlorhexidine Susceptibility during Chlorhexidine Use.

Authors:  Ryan C Johnson; Carey D Schlett; Katrina Crawford; Jeffrey B Lanier; D Scott Merrell; Michael W Ellis
Journal:  J Clin Microbiol       Date:  2015-08-19       Impact factor: 5.948

6.  Staphylococcus aureus Colonization and Strain Type at Various Body Sites among Patients with a Closed Abscess and Uninfected Controls at U.S. Emergency Departments.

Authors:  Valerie S Albrecht; Brandi M Limbago; Gregory J Moran; Anusha Krishnadasan; Rachel J Gorwitz; Linda K McDougal; David A Talan
Journal:  J Clin Microbiol       Date:  2015-08-19       Impact factor: 5.948

7.  Characterization of a novel thermostable nuclease homolog (NucM) in a highly divergent Staphylococcus aureus clade.

Authors:  Frieder Schaumburg; Maude Pauly; Grit Schubert; Adebayo Shittu; Steven Tong; Fabian Leendertz; Georg Peters; Karsten Becker
Journal:  J Clin Microbiol       Date:  2014-08-20       Impact factor: 5.948

8.  Frequent use of chlorhexidine-based body wash associated with a reduction in methicillin-resistant Staphylococcus aureus nasal colonization among military trainees.

Authors:  Eugene V Millar; Wei-Ju Chen; Carey D Schlett; Tianyuan Cui; Katrina B Crawford; Jeffrey B Lanier; David R Tribble; Michael W Ellis
Journal:  Antimicrob Agents Chemother       Date:  2014-11-24       Impact factor: 5.191

9.  Evaluation of the impact of direct plating, broth enrichment, and specimen source on recovery and diversity of methicillin-resistant Staphylococcus aureus isolates among HIV-infected outpatients.

Authors:  S K McAllister; V S Albrecht; G E Fosheim; H K Lowery; P J Peters; R Gorwitz; J L Guest; J Hageman; R Mindley; L K McDougal; D Rimland; B Limbago
Journal:  J Clin Microbiol       Date:  2011-10-12       Impact factor: 5.948

10.  Prevalence of nasal colonization and strain concordance in patients with community-associated Staphylococcus aureus skin and soft-tissue infections.

Authors:  Michael W Ellis; Carey D Schlett; Eugene V Millar; Katrina B Crawford; Tianyuan Cui; Jeffrey B Lanier; David R Tribble
Journal:  Infect Control Hosp Epidemiol       Date:  2014-08-19       Impact factor: 3.254
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