Takafumi Toyohara1, Takehiro Suzuki1, Yasutoshi Akiyama1, Daisuke Yoshihara2, Yoichi Takeuchi1,3, Eikan Mishima1, Koichi Kikuchi1, Chitose Suzuki1, Masayuki Tanemoto1, Sadayoshi Ito1, Shizuko Nagao2, Tomoyoshi Soga4, Takaaki Abe5,6,7. 1. Division of Nephrology, Endocrinology and Vascular Medicine, Tohoku University, Graduate School of Medicine, Sendai, 980-8574, Japan. 2. Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, 470-1192, Japan. 3. Department of Clinical Biology and Hormonal Regulation, Tohoku University, Graduate School of Medicine, Sendai, 980-8574, Japan. 4. Institute for Advanced Biosciences, Keio University, Tsuruoka, 997-0052, Japan. 5. Division of Nephrology, Endocrinology and Vascular Medicine, Tohoku University, Graduate School of Medicine, Sendai, 980-8574, Japan. takaabe@med.tohoku.ac.jp. 6. Division of Medical Science, Tohoku University Graduate School of Biomedical Engineering, Sendai, 980-8574, Japan. takaabe@med.tohoku.ac.jp. 7. Department of Clinical Biology and Hormonal Regulation, Tohoku University, Graduate School of Medicine, Sendai, 980-8574, Japan. takaabe@med.tohoku.ac.jp.
Abstract
BACKGROUND: Autosomal dominant polycystic kidney disease (ADPKD) is an inherited systemic disease characterized by renal cyst expansion, resulting in renal failure. With the progression of renal damage, the accumulation of uremic compounds is recently reported to subsequently cause further renal damage and hypertension. Finding uremic toxins and sensitive markers for detecting the early stage of ADPKD is necessary to clarify its pathophysiological process and to prevent its progression. The aim of this study was to analyze the profile of uremic retention solutes of ADPKD by capillary electrophoresis-mass spectrometry (CE-MS) using the Han:SPRD rat model. METHODS: Two hundred and ninety-seven cations and 190 anions were comprehensively analyzed by CE-MS in Han:SPRD rats and control rats. RESULTS: We found 21 cations and 19 anions that accumulated significantly in the heterozygous (Cy/+) ADPKD rat model compared with control rats. Among the compounds, increases in 5-methyl-2'-deoxycytidine, glucosamine, ectoine, allantoate, α-hydroxybenzoate, phenaceturate and 3-phenylpropionate and decreases in 2-deoxycytidine, decanoate and 10-hydroxydecanoate were newly identified in the ADPKD Cy/+ rats. CONCLUSION: We identified uremic retention solutes in ADPKD Cy/+ rats. Compounds related to ADPKD could be useful markers for detecting the early stage of ADPKD.
BACKGROUND:Autosomal dominant polycystic kidney disease (ADPKD) is an inherited systemic disease characterized by renal cyst expansion, resulting in renal failure. With the progression of renal damage, the accumulation of uremic compounds is recently reported to subsequently cause further renal damage and hypertension. Finding uremic toxins and sensitive markers for detecting the early stage of ADPKD is necessary to clarify its pathophysiological process and to prevent its progression. The aim of this study was to analyze the profile of uremic retention solutes of ADPKD by capillary electrophoresis-mass spectrometry (CE-MS) using the Han:SPRD rat model. METHODS: Two hundred and ninety-seven cations and 190 anions were comprehensively analyzed by CE-MS in Han:SPRD rats and control rats. RESULTS: We found 21 cations and 19 anions that accumulated significantly in the heterozygous (Cy/+) ADPKD rat model compared with control rats. Among the compounds, increases in 5-methyl-2'-deoxycytidine, glucosamine, ectoine, allantoate, α-hydroxybenzoate, phenaceturate and 3-phenylpropionate and decreases in 2-deoxycytidine, decanoate and 10-hydroxydecanoate were newly identified in the ADPKD Cy/+ rats. CONCLUSION: We identified uremic retention solutes in ADPKD Cy/+ rats. Compounds related to ADPKD could be useful markers for detecting the early stage of ADPKD.
Authors: S Nagao; T Ushijima; M Kasahara; T Yamaguchi; M Kusaka; J Matsuda; M Nagao; H Takahashi Journal: Biochem Genet Date: 1999-08 Impact factor: 1.890
Authors: Victoria Cachofeiro; Marian Goicochea; Soledad García de Vinuesa; Pilar Oubiña; Vicente Lahera; José Luño Journal: Kidney Int Suppl Date: 2008-12 Impact factor: 10.545