BACKGROUND: The greater omentum with its vascularization and blood flow has been considered as a location for islet transplantation; however, there is a need to provide a controlled and protected site for the islets within the omentum that would be applicable to donor islets and future stem cell technologies. Here we describe the use of a novel device implanted within the omentum with a subcutaneous delivery port that offers an environment for donor islets. METHODS: A prototype cell pouch device was wrapped in the greater omentum and an islet implantation port was exposed subcutaneously in diabetic Lewis rats. After tissue growth throughout the device, islet isografts were implanted and long-term glucose control was evaluated. RESULTS: By using this technique, 7 of 10 diabetic rat recipients showed long-term normal blood glucose levels after minimal islet dose transplants. Histologic assessment revealed collagen formation and vascularization within the device. CONCLUSIONS: The implanted device assessed using this technique provides a safe and efficacious environment for the support of pancreatic islets contained within a removable device as a cell therapy in a highly vascularized setting.
BACKGROUND: The greater omentum with its vascularization and blood flow has been considered as a location for islet transplantation; however, there is a need to provide a controlled and protected site for the islets within the omentum that would be applicable to donor islets and future stem cell technologies. Here we describe the use of a novel device implanted within the omentum with a subcutaneous delivery port that offers an environment for donor islets. METHODS: A prototype cell pouch device was wrapped in the greater omentum and an islet implantation port was exposed subcutaneously in diabetic Lewisrats. After tissue growth throughout the device, islet isografts were implanted and long-term glucose control was evaluated. RESULTS: By using this technique, 7 of 10 diabeticrat recipients showed long-term normal blood glucose levels after minimal islet dose transplants. Histologic assessment revealed collagen formation and vascularization within the device. CONCLUSIONS: The implanted device assessed using this technique provides a safe and efficacious environment for the support of pancreatic islets contained within a removable device as a cell therapy in a highly vascularized setting.
Authors: Rajesh Pareta; John P McQuilling; Sivanandane Sittadjody; Randy Jenkins; Stephen Bowden; Giuseppe Orlando; Alan C Farney; Eric M Brey; Emmanuel C Opara Journal: Pancreas Date: 2014-05 Impact factor: 3.327
Authors: Alexander U Ernst; Daniel T Bowers; Long-Hai Wang; Kaavian Shariati; Mitchell D Plesser; Natalie K Brown; Tigran Mehrabyan; Minglin Ma Journal: Adv Drug Deliv Rev Date: 2019-02-02 Impact factor: 15.470
Authors: Naresh Kasoju; Dana Kubies; Marta M Kumorek; Jan Kříž; Eva Fábryová; Lud'ka Machová; Jana Kovářová; František Rypáček Journal: PLoS One Date: 2014-10-02 Impact factor: 3.240
Authors: Alisa M White; James G Shamul; Jiangsheng Xu; Samantha Stewart; Jonathan S Bromberg; Xiaoming He Journal: ACS Biomater Sci Eng Date: 2019-12-02
Authors: Eileen Pedraza; Ann-Christina Brady; Christopher A Fraker; R Damaris Molano; Steven Sukert; Dora M Berman; Norma S Kenyon; Antonello Pileggi; Camillo Ricordi; Cherie L Stabler Journal: Cell Transplant Date: 2012-10-02 Impact factor: 4.064