Literature DB >> 2167443

Overexpression of a leaderless form of yeast cytochrome c oxidase subunit Va circumvents the requirement for a leader peptide in mitochondrial import.

L K Dircks1, R O Poyton.   

Abstract

Subunit Va of Saccharomyces cerevisiae cytochrome c oxidase is a nucleus-encoded mitochondrial protein that is derived from a precursor with a 20-residue leader peptide. We previously reported that this leader peptide is required for import of subunit Va into mitochondria in vivo (S. M. Glaser, C. E. Trueblood, L. K. Dircks, R. O. Poyton, and M. G. Cumsky, J. Cell. Biochem. 36:275-278, 1988). Here we show that overproduction of a leaderless form of subunit Va circumvents the leader peptide requirement for import into mitochondria in vivo.

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Year:  1990        PMID： 2167443      PMCID： PMC361129          DOI： 10.1128/mcb.10.9.4984-4986.1990

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  20 in total

1.  Mitochondrial protein import. Bypass of proteinaceous surface receptors can occur with low specificity and efficiency.

Authors:  R Pfaller; N Pfanner; W Neupert
Journal:  J Biol Chem       Date:  1989-01-05       Impact factor: 5.157

2.  Regulation of mitochondrial protein synthesis by cytoplasmic proteins.

Authors:  R O Poyton; J Kavanagh
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

3.  Yeast/E. coli shuttle vectors with multiple unique restriction sites.

Authors:  J E Hill; A M Myers; T J Koerner; A Tzagoloff
Journal:  Yeast       Date:  1986-09       Impact factor: 3.239

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  The yeast F1-ATPase beta subunit precursor contains functionally redundant mitochondrial protein import information.

Authors:  D M Bedwell; D J Klionsky; S D Emr
Journal:  Mol Cell Biol       Date:  1987-11       Impact factor: 4.272

7.  The hydrophobic moment detects periodicity in protein hydrophobicity.

Authors:  D Eisenberg; R M Weiss; T C Terwilliger
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

8.  Sequences from a prokaryotic genome or the mouse dihydrofolate reductase gene can restore the import of a truncated precursor protein into yeast mitochondria.

Authors:  A Baker; G Schatz
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

9.  Rapid method for isolation and screening of cytochrome c oxidase-deficient mutants of Saccharomyces cerevisiae.

Authors:  J E McEwen; V L Cameron; R O Poyton
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

10.  Sequence and structural requirements of a mitochondrial protein import signal defined by saturation cassette mutagenesis.

Authors:  D M Bedwell; S A Strobel; K Yun; G D Jongeward; S D Emr
Journal:  Mol Cell Biol       Date:  1989-03       Impact factor: 4.272
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  5 in total

1.  The NAM8 gene in Saccharomyces cerevisiae encodes a protein with putative RNA binding motifs and acts as a suppressor of mitochondrial splicing deficiencies when overexpressed.

Authors:  K Ekwall; M Kermorgant; G Dujardin; O Groudinsky; P P Slonimski
Journal:  Mol Gen Genet       Date:  1992-05

2.  Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase.

Authors:  Rudolf Mitterbauer; Thomas Karl; Gerhard Adam
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

3.  HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA synthetase of Saccharomyces cerevisiae: mutations alter the specificity of compartmentation.

Authors:  M I Chiu; T L Mason; G R Fink
Journal:  Genetics       Date:  1992-12       Impact factor: 4.562

4.  Interactions among three proteins that specifically activate translation of the mitochondrial COX3 mRNA in Saccharomyces cerevisiae.

Authors:  N G Brown; M C Costanzo; T D Fox
Journal:  Mol Cell Biol       Date:  1994-02       Impact factor: 4.272

5.  Functional analysis of a recently originating, atypical presequence: mitochondrial import and processing of GUS fusion proteins in transgenic tobacco and yeast.

Authors:  J Huang; C S Levings
Journal:  Plant Mol Biol       Date:  1995-11       Impact factor: 4.076
  5 in total

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