Literature DB >> 2167432

Recombinant transforming growth factor type beta 3: biological activities and receptor-binding properties in isolated bone cells.

P ten Dijke1, K K Iwata, C Goddard, C Pieler, E Canalis, T L McCarthy, M Centrella.   

Abstract

We have recently cloned the cDNA for transforming growth factor type beta 3 (TGF-beta 3), a new member of the TGF-beta gene family. We examined the biological effects of recombinant TGF-beta 3 protein in osteoblast-enriched bone cell cultures. In this report we demonstrate that TGF-beta 3 is a potent regulator of functions associated with bone formation, i.e., mitogenesis, collagen synthesis, and alkaline phosphatase activity. In a direct comparison between TGF-beta 3 and TGF-beta 1, TGF-beta 3 appeared to be three- to fivefold more potent than TGF-beta 1. Our cross-linking experiments with iodinated TGF-beta showed that in osteoblast-enriched bone cell cultures, both TGF-beta 3 and TGF-beta 1 associated with the same three cell surface binding sites. Scatchard analysis of receptor competition studies indicated the presence of high-affinity binding sites for TGF-beta 3 in the picomolar range. TGF-beta 3 showed an approximately fourfold-higher apparent affinity than TGF-beta 1 in overall binding.

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Year:  1990        PMID： 2167432      PMCID： PMC361033          DOI： 10.1128/mcb.10.9.4473-4479.1990

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  30 in total

1.  Molecular characterization of transforming growth factor type beta 3.

Authors:  P ten Dijke; K K Iwata; M Thorikay; J Schwedes; A Stewart; C Pieler
Journal:  Ann N Y Acad Sci       Date:  1990       Impact factor: 5.691

Review 2.  Skeletal tissue and transforming growth factor beta.

Authors:  M Centrella; T L McCarthy; E Canalis
Journal:  FASEB J       Date:  1988-12       Impact factor: 5.191

3.  Transforming growth factor-beta inhibition of epithelial cell proliferation linked to the expression of a 53-kDa membrane receptor.

Authors:  F T Boyd; J Massagué
Journal:  J Biol Chem       Date:  1989-02-05       Impact factor: 5.157

4.  Cellular receptors for type beta transforming growth factor. Ligand binding and affinity labeling in human and rodent cell lines.

Authors:  J Massagué; B Like
Journal:  J Biol Chem       Date:  1985-03-10       Impact factor: 5.157

5.  Further biochemical and molecular characterization of primary rat parietal bone cell cultures.

Authors:  T L McCarthy; M Centrella; E Canalis
Journal:  J Bone Miner Res       Date:  1988-08       Impact factor: 6.741

6.  Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.

Authors:  B Peterkofsky; R Diegelmann
Journal:  Biochemistry       Date:  1971-03-16       Impact factor: 3.162

7.  Modulation of type beta transforming growth factor activity in bone cultures by osteotropic hormones.

Authors:  J Pfeilschifter; G R Mundy
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

8.  Alpha and beta human transforming growth factors stimulate prostaglandin production and bone resorption in cultured mouse calvaria.

Authors:  A H Tashjian; E F Voelkel; M Lazzaro; F R Singer; A B Roberts; R Derynck; M E Winkler; L Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

9.  Mesoderm induction in amphibians: the role of TGF-beta 2-like factors.

Authors:  F Rosa; A B Roberts; D Danielpour; L L Dart; M B Sporn; I B Dawid
Journal:  Science       Date:  1988-02-12       Impact factor: 47.728

10.  Distribution and modulation of the cellular receptor for transforming growth factor-beta.

Authors:  L M Wakefield; D M Smith; T Masui; C C Harris; M B Sporn
Journal:  J Cell Biol       Date:  1987-08       Impact factor: 10.539
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  17 in total

1.  Transforming growth factor beta one (TGF-beta 1) enhancement of the chondrocytic phenotype in aged perichondrial cells: an in vitro study.

Authors:  M C Lee; R S Goomer; K Takahashi; F L Harwood; M Amiel; D Amiel
Journal:  Iowa Orthop J       Date:  2000

2.  Increased ΤGF-β3 in primary biliary cirrhosis: an abnormality related to pathogenesis?

Authors:  Argyro Voumvouraki; Mairi Koulentaki; Maria Tzardi; Ourania Sfakianaki; Penelope Manousou; George Notas; Elias Kouroumalis
Journal:  World J Gastroenterol       Date:  2010-10-28       Impact factor: 5.742

3.  Indomethacin suppresses the anti-proliferative effects of transforming growth factor-beta isoforms on fibroblast cell cultures.

Authors:  R J McAnulty; N A Hernández-Rodriguez; S E Mutsaers; R K Coker; G J Laurent
Journal:  Biochem J       Date:  1997-02-01       Impact factor: 3.857

4.  Transforming growth factors-beta 1, -beta 2, and -beta 3 stimulate fibroblast procollagen production in vitro but are differentially expressed during bleomycin-induced lung fibrosis.

Authors:  R K Coker; G J Laurent; S Shahzeidi; P A Lympany; R M du Bois; P K Jeffery; R J McAnulty
Journal:  Am J Pathol       Date:  1997-03       Impact factor: 4.307

5.  Effects of TGF-beta3 and preculture period of osteogenic cells on the chondrogenic differentiation of rabbit marrow mesenchymal stem cells encapsulated in a bilayered hydrogel composite.

Authors:  X Guo; J Liao; H Park; A Saraf; R M Raphael; Y Tabata; F K Kasper; A G Mikos
Journal:  Acta Biomater       Date:  2010-03-01       Impact factor: 8.947

6.  Transforming growth factor beta (TGFbeta) mediates Schwann cell death in vitro and in vivo: examination of c-Jun activation, interactions with survival signals, and the relationship of TGFbeta-mediated death to Schwann cell differentiation.

Authors:  D B Parkinson; Z Dong; H Bunting; J Whitfield; C Meier; H Marie; R Mirsky; K R Jessen
Journal:  J Neurosci       Date:  2001-11-01       Impact factor: 6.167

7.  Activin-A binding and biochemical effects in osteoblast-enriched cultures from fetal-rat parietal bone.

Authors:  M Centrella; T L McCarthy; E Canalis
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

8.  Roles of stromal cell RANKL, OPG, and M-CSF expression in biphasic TGF-beta regulation of osteoclast differentiation.

Authors:  Mary Karst; Genevieve Gorny; Rachelle J Sells Galvin; Merry Jo Oursler
Journal:  J Cell Physiol       Date:  2004-07       Impact factor: 6.384

Review 9.  TGF-beta3 and cancer: a review.

Authors:  H G Laverty; L M Wakefield; N L Occleston; S O'Kane; M W J Ferguson
Journal:  Cytokine Growth Factor Rev       Date:  2009-08-04       Impact factor: 7.638

10.  The urokinase plasminogen activator (u-PA) and its inhibitor (PAI-1) in embryo-fetal bone formation in the human: an immunohistochemical study.

Authors:  C Häckel; K Radig; I Röse; A Roessner
Journal:  Anat Embryol (Berl)       Date:  1995-10
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