PURPOSE: The purpose of this study was to clarify the day-to-day variations of salivary cortisol to discuss the appropriate timing for collecting saliva in a day for stress evaluation. MATERIALS AND METHODS: Saliva samples from twelve healthy adults were collected by means of Salivette(®) (SARSTEDT Co.) three times per day, immediately after awakening (Time1), 6:00 PM (Time2) and 30 min before going to bed (Time3), on 5 nonconsecutive days. Cortisol concentration was measured using a radioimmunoassay. RESULTS: Salivary cortisol concentration showed the larger value at Time1 than that at Time2. The mean value of %CV for day-to-day variation showed the smallest value at Time1 (mean: 43.1%) and the largest value at Time3 (mean: 100.3%). There were significant differences in %CV value between Time1 and Time3, Time2 (mean: 64.1%) and Time3. CONCLUSIONS: There are considerable day-to-day variations in salivary cortisol concentration measurements. From the viewpoint of day-to-day variation, collecting saliva immediately after awakening in the morning is thought to be more advantageous for evaluating cortisol concentration.
PURPOSE: The purpose of this study was to clarify the day-to-day variations of salivary cortisol to discuss the appropriate timing for collecting saliva in a day for stress evaluation. MATERIALS AND METHODS: Saliva samples from twelve healthy adults were collected by means of Salivette(®) (SARSTEDT Co.) three times per day, immediately after awakening (Time1), 6:00 PM (Time2) and 30 min before going to bed (Time3), on 5 nonconsecutive days. Cortisol concentration was measured using a radioimmunoassay. RESULTS: Salivary cortisol concentration showed the larger value at Time1 than that at Time2. The mean value of %CV for day-to-day variation showed the smallest value at Time1 (mean: 43.1%) and the largest value at Time3 (mean: 100.3%). There were significant differences in %CV value between Time1 and Time3, Time2 (mean: 64.1%) and Time3. CONCLUSIONS: There are considerable day-to-day variations in salivary cortisol concentration measurements. From the viewpoint of day-to-day variation, collecting saliva immediately after awakening in the morning is thought to be more advantageous for evaluating cortisol concentration.
Authors: John Newell-Price; Rosario Pivonello; Antoine Tabarin; Maria Fleseriu; Przemysław Witek; Mônica R Gadelha; Stephan Petersenn; Libuse Tauchmanova; Shoba Ravichandran; Pritam Gupta; André Lacroix; Beverly M K Biller Journal: Eur J Endocrinol Date: 2020-02 Impact factor: 6.664
Authors: James W Findling; Maria Fleseriu; John Newell-Price; Stephan Petersenn; Rosario Pivonello; Albert Kandra; Alberto M Pedroncelli; Beverly M K Biller Journal: Endocrine Date: 2016-05-21 Impact factor: 3.633