BACKGROUND: White blood cells (WBCs) collected from hematopoietic stem cell transplant donors are often given to the recipient to speed immune recovery or treat disease relapse. The postthaw recovery and viability of cryopreserved donor WBCs, stored for as long as 7 years, were assessed. STUDY DESIGN AND METHODS: Total nucleated cell (TNC) cell recovery, CD3+ cell recovery, and TNC viability were measured in 311 clinical donor WBC products: 168 products were unmanipulated or minimally manipulated and 143 products were extensively manipulated. An additional 45 products were selected because they were stored for a longer duration; these were tested using both standard methods and global transcriptional analysis. All products were cryopreserved in 5% dimethyl sulfoxide (DMSO) plus 6% pentastarch and stored in liquid nitrogen. RESULTS: The mean duration of storage of the 311 products was 143 days. Their TNC recovery was 92 ± 17%, CD3+ cell recovery was 76 ± 19%, and the TNC viability was 84 ± 6%. Duration of storage had no effect on TNC recovery, CD3+ cell recovery, or TNC viability of the 311 products. The mean duration of storage of the long-term stored products was 5.2 years; their TNC recovery (93 ± 14%) and the TNC viability (78 ± 13%) did not differ from the 311 products, but their CD3 cell recovery was greater (86 ± 22%; p = 0.0042). Gene expression profiles of the long-term-stored products revealed no differences due to storage duration. CONCLUSIONS: Donor WBC products cryopreserved in 5% DMSO and 6% pentastarch can be stored in liquid nitrogen for at least 7 years.
BACKGROUND: White blood cells (WBCs) collected from hematopoietic stem cell transplant donors are often given to the recipient to speed immune recovery or treat disease relapse. The postthaw recovery and viability of cryopreserved donor WBCs, stored for as long as 7 years, were assessed. STUDY DESIGN AND METHODS: Total nucleated cell (TNC) cell recovery, CD3+ cell recovery, and TNC viability were measured in 311 clinical donor WBC products: 168 products were unmanipulated or minimally manipulated and 143 products were extensively manipulated. An additional 45 products were selected because they were stored for a longer duration; these were tested using both standard methods and global transcriptional analysis. All products were cryopreserved in 5% dimethyl sulfoxide (DMSO) plus 6% pentastarch and stored in liquid nitrogen. RESULTS: The mean duration of storage of the 311 products was 143 days. Their TNC recovery was 92 ± 17%, CD3+ cell recovery was 76 ± 19%, and the TNC viability was 84 ± 6%. Duration of storage had no effect on TNC recovery, CD3+ cell recovery, or TNC viability of the 311 products. The mean duration of storage of the long-term stored products was 5.2 years; their TNC recovery (93 ± 14%) and the TNC viability (78 ± 13%) did not differ from the 311 products, but their CD3 cell recovery was greater (86 ± 22%; p = 0.0042). Gene expression profiles of the long-term-stored products revealed no differences due to storage duration. CONCLUSIONS:Donor WBC products cryopreserved in 5% DMSO and 6% pentastarch can be stored in liquid nitrogen for at least 7 years.
Authors: Elizabeth M Kang; Ellen M Areman; Virginia David-Ocampo; Courtney Fitzhugh; Mary E Link; Elizabeth J Read; Susan F Leitman; Griffin P Rodgers; John F Tisdale Journal: Blood Date: 2002-02-01 Impact factor: 22.113
Authors: H M Khuu; H Cowley; V David-Ocampo; C S Carter; C Kasten-Sportes; A S Wayne; S R Solomon; M R Bishop; R M Childs; E J Read Journal: Cytotherapy Date: 2002 Impact factor: 5.414
Authors: Ping Jin; Ena Wang; Jiaqiang Ren; Richard Childs; Jeong Won Shin; Hanh Khuu; Francesco M Marincola; David F Stroncek Journal: J Transl Med Date: 2008-07-22 Impact factor: 5.531
Authors: Virginia Fisher; Hanh Khuu; Virginia David-Ocampo; Karen Byrne; Steven Pavletic; Michael Bishop; Daniel H Fowler; A John Barrett; David F Stroncek Journal: Transfusion Date: 2013-10-10 Impact factor: 3.157
Authors: Rebecca E Burnham; Donald Tope; Gianna Branella; Erich Williams; Christopher B Doering; H Trent Spencer Journal: Cryobiology Date: 2021-01-21 Impact factor: 2.487
Authors: Sean M Hughes; April L Ferre; Sarah E Yandura; Cory Shetler; Chris A R Baker; Fernanda Calienes; Claire N Levy; Rena D Astronomo; Zhiquan Shu; Gretchen M Lentz; Michael Fialkow; Anna C Kirby; M Juliana McElrath; Elizabeth Sinclair; Lisa C Rohan; Peter L Anderson; Barbara L Shacklett; Charlene S Dezzutti; Dayong Gao; Florian Hladik Journal: PLoS One Date: 2018-07-30 Impact factor: 3.240
Authors: Sean M Hughes; Zhiquan Shu; Claire N Levy; April L Ferre; Heather Hartig; Cifeng Fang; Gretchen Lentz; Michael Fialkow; Anna C Kirby; Kristina M Adams Waldorf; Ronald S Veazey; Anja Germann; Hagen von Briesen; M Juliana McElrath; Charlene S Dezzutti; Elizabeth Sinclair; Chris A R Baker; Barbara L Shacklett; Dayong Gao; Florian Hladik Journal: PLoS One Date: 2016-05-27 Impact factor: 3.752
Authors: Daniel Missailidis; Oana Sanislav; Claire Y Allan; Sarah J Annesley; Paul R Fisher Journal: Int J Mol Sci Date: 2020-02-08 Impact factor: 5.923