BACKGROUND: Autologous stem cell transplantation(ASCT) is used in the treatment of several malignancies.Harvesting sufficient peripheral blood progenitor cells (PBPCs) for a potential second autotransplantation at the time of relapse several years after diagnosis is becoming an increasingly common practice. STUDY DESIGN AND METHODS: Cryopreserved PBPCs were prepared with different concentrations of dimethyl sulfoxide (DMSO; 2, 4, 5, and 10%) and stored for at least 5 years before the recovery of CD34+ cells and various T- and natural killer (NK)-cell subsets were analyzed by flow cytometry. Furthermore,clinical variables for myeloma patients having a second autotransplantation with long-term-stored autografts were evaluated. RESULTS: The number of viable CD34+ cells in longterm-stored grafts was higher when autografts were cryopreserved with 4 or 5% than with 2 and 10%DMSO. The number of viable CD34+ cells was reduced by 13.9% after 5 years of cryostorage in 5% DMSO.Lymphocyte viability was also higher with 4 or 5%DMSO. However, the frequencies of several T-cell subsets showed DMSO-dependent differences,whereas NK-cell subsets did not. Furthermore, after a second autotransplantation with long-term-stored PBPC grafts at the time of myeloma relapse (median storage time, 42 months) all 17 patients reached neutrophil counts exceeding 0.5 x 10⁹/L and platelet counts exceeding 20 x 10⁹/L within 15 days. There was no difference in engraftment between patients receiving autografts preserved with 5 and 10% DMSO. CONCLUSION: PBPC autografts can safely be stored for at least 5 years in 5% DMSO and used for ASCT.
BACKGROUND: Autologous stem cell transplantation(ASCT) is used in the treatment of several malignancies.Harvesting sufficient peripheral blood progenitor cells (PBPCs) for a potential second autotransplantation at the time of relapse several years after diagnosis is becoming an increasingly common practice. STUDY DESIGN AND METHODS: Cryopreserved PBPCs were prepared with different concentrations of dimethyl sulfoxide (DMSO; 2, 4, 5, and 10%) and stored for at least 5 years before the recovery of CD34+ cells and various T- and natural killer (NK)-cell subsets were analyzed by flow cytometry. Furthermore,clinical variables for myelomapatients having a second autotransplantation with long-term-stored autografts were evaluated. RESULTS: The number of viable CD34+ cells in longterm-stored grafts was higher when autografts were cryopreserved with 4 or 5% than with 2 and 10%DMSO. The number of viable CD34+ cells was reduced by 13.9% after 5 years of cryostorage in 5% DMSO.Lymphocyte viability was also higher with 4 or 5%DMSO. However, the frequencies of several T-cell subsets showed DMSO-dependent differences,whereas NK-cell subsets did not. Furthermore, after a second autotransplantation with long-term-stored PBPC grafts at the time of myeloma relapse (median storage time, 42 months) all 17 patients reached neutrophil counts exceeding 0.5 x 10⁹/L and platelet counts exceeding 20 x 10⁹/L within 15 days. There was no difference in engraftment between patients receiving autografts preserved with 5 and 10% DMSO. CONCLUSION: PBPC autografts can safely be stored for at least 5 years in 5% DMSO and used for ASCT.
Authors: T Shima; H Iwasaki; T Yamauchi; M Kadowaki; M Kiyosuke; T Mochimaru; K Takenaka; T Miyamoto; K Akashi; T Teshima Journal: Bone Marrow Transplant Date: 2015-06-22 Impact factor: 5.483
Authors: Virginia Fisher; Hanh Khuu; Virginia David-Ocampo; Karen Byrne; Steven Pavletic; Michael Bishop; Daniel H Fowler; A John Barrett; David F Stroncek Journal: Transfusion Date: 2013-10-10 Impact factor: 3.157
Authors: John Underwood; Mahvish Rahim; Carijo West; Rebecca Britton; Elaine Skipworth; Vicki Graves; Steven Sexton; Hillary Harris; Dave Schwering; Anthony Sinn; Karen E Pollok; Kent A Robertson; W Scott Goebel; Kerry M Hege Journal: World J Stem Cells Date: 2020-05-26 Impact factor: 5.326
Authors: Rebecca E Burnham; Donald Tope; Gianna Branella; Erich Williams; Christopher B Doering; H Trent Spencer Journal: Cryobiology Date: 2021-01-21 Impact factor: 2.487
Authors: Tor Henrik Anderson Tvedt; Guro K Melve; Galina Tsykunova; Aymen Bushra Ahmed; Annette K Brenner; Øystein Bruserud Journal: Int J Mol Sci Date: 2018-09-22 Impact factor: 5.923