Effect of toxic doses of cholecalciferol (vitamin D3) on alkaline phosphatase levels in serum and gingival tissues of rats.

Toxic doses of cholecalciferol (vitamin D3) were injected subcutaneously (25 micrograms/day) into rats for 5 weeks, causing a significant increase of serum alkaline phosphatase (ALP) levels (p less than 0.01). Gingival tissue alkaline phosphatase levels were also elevated (p less than 0.01). On the other hand, a significant difference was detected between serum calcium levels in experimental and control groups (p less than 0.02).