Literature DB >> 21643439

Fc-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING.

Robabeh Rezaeipoor1, Renu John, Steven G Adie, Eric J Chaney, Marina Marjanovic, Amy L Oldenburg, Stephanie A Rinne, Stephen A Boppart.   

Abstract

In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

Entities:  

Year:  2009        PMID： 21643439      PMCID： PMC3105601          DOI： 10.1142/S1793545809000693

Source DB:  PubMed          Journal:  J Innov Opt Health Sci


  38 in total

Review 1.  Molecular imaging in vivo with PET and SPECT.

Authors:  G D Luker; D Piwnica-Worms
Journal:  Acad Radiol       Date:  2001-01       Impact factor: 3.173

2.  Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping.

Authors:  Sungjee Kim; Yong Taik Lim; Edward G Soltesz; Alec M De Grand; Jaihyoung Lee; Akira Nakayama; J Anthony Parker; Tomislav Mihaljevic; Rita G Laurence; Delphine M Dor; Lawrence H Cohn; Moungi G Bawendi; John V Frangioni
Journal:  Nat Biotechnol       Date:  2003-12-07       Impact factor: 54.908

3.  Immunotargeted nanoshells for integrated cancer imaging and therapy.

Authors:  Christopher Loo; Amanda Lowery; Naomi Halas; Jennifer West; Rebekah Drezek
Journal:  Nano Lett       Date:  2005-04       Impact factor: 11.189

Review 4.  Optical probes and techniques for molecular contrast enhancement in coherence imaging.

Authors:  Stephen A Boppart; Amy L Oldenburg; Chenyang Xu; Daniel L Marks
Journal:  J Biomed Opt       Date:  2005 Jul-Aug       Impact factor: 3.170

5.  Magnetomotive contrast for in vivo optical coherence tomography.

Authors:  Amy Oldenburg; Farah Toublan; Kenneth Suslick; Alexander Wei; Stephen Boppart
Journal:  Opt Express       Date:  2005-08-22       Impact factor: 3.894

6.  Biomimetic amplification of nanoparticle homing to tumors.

Authors:  Dmitri Simberg; Tasmia Duza; Ji Ho Park; Markus Essler; Jan Pilch; Lianglin Zhang; Austin M Derfus; Meng Yang; Robert M Hoffman; Sangeeta Bhatia; Michael J Sailor; Erkki Ruoslahti
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-10       Impact factor: 11.205

7.  Multifunctional polymeric micelles as cancer-targeted, MRI-ultrasensitive drug delivery systems.

Authors:  Norased Nasongkla; Erik Bey; Jimin Ren; Hua Ai; Chalermchai Khemtong; Jagadeesh Setti Guthi; Shook-Fong Chin; A Dean Sherry; David A Boothman; Jinming Gao
Journal:  Nano Lett       Date:  2006-11       Impact factor: 11.189

8.  Biodegradation of magnetite dextran nanoparticles in the rat. A histologic and biophysical study.

Authors:  E Okon; D Pouliquen; P Okon; Z V Kovaleva; T P Stepanova; S G Lavit; B N Kudryavtsev; P Jallet
Journal:  Lab Invest       Date:  1994-12       Impact factor: 5.662

9.  Water-soluble quantum dots for multiphoton fluorescence imaging in vivo.

Authors:  Daniel R Larson; Warren R Zipfel; Rebecca M Williams; Stephen W Clark; Marcel P Bruchez; Frank W Wise; Watt W Webb
Journal:  Science       Date:  2003-05-30       Impact factor: 47.728

10.  Distinct ErbB-2 coupled signaling pathways promote mammary tumors with unique pathologic and transcriptional profiles.

Authors:  Babette Schade; Sonya H L Lam; Daniela Cernea; Virginie Sanguin-Gendreau; Robert D Cardiff; Boonim L Jung; Michael Hallett; William J Muller
Journal:  Cancer Res       Date:  2007-08-15       Impact factor: 12.701
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  5 in total

Review 1.  Magnetomotive molecular nanoprobes.

Authors:  Renu John; Stephen A Boppart
Journal:  Curr Med Chem       Date:  2011       Impact factor: 4.530

2.  Magnetomotive Optical Coherence Elastography for Magnetic Hyperthermia Dosimetry Based on Dynamic Tissue Biomechanics.

Authors:  Pin-Chieh Huang; Paritosh Pande; Adeel Ahmad; Marina Marjanovic; Darold R Spillman; Boris Odintsov; Stephen A Boppart
Journal:  IEEE J Sel Top Quantum Electron       Date:  2015-12-17       Impact factor: 4.544

3.  In vivo magnetomotive optical molecular imaging using targeted magnetic nanoprobes.

Authors:  Renu John; Robabeh Rezaeipoor; Steven G Adie; Eric J Chaney; Amy L Oldenburg; Marina Marjanovic; Justin P Haldar; Bradley P Sutton; Stephen A Boppart
Journal:  Proc Natl Acad Sci U S A       Date:  2010-04-19       Impact factor: 11.205

Review 4.  The Protein Corona as a Confounding Variable of Nanoparticle-Mediated Targeted Vaccine Delivery.

Authors:  Matthias Bros; Lutz Nuhn; Johanna Simon; Lorna Moll; Volker Mailänder; Katharina Landfester; Stephan Grabbe
Journal:  Front Immunol       Date:  2018-08-02       Impact factor: 7.561

5.  Quantitative FRET imaging to visualize the invasiveness of live breast cancer cells.

Authors:  Shaoying Lu; Yi Wang; He Huang; Yijia Pan; Eric J Chaney; Stephen A Boppart; Howard Ozer; Alex Y Strongin; Yingxiao Wang
Journal:  PLoS One       Date:  2013-03-13       Impact factor: 3.240
  5 in total

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