Combined Ion Mobility/Time-of-Flight Mass Spectrometry Study of Electrospray-Generated Ions.

A commercially available ion mobility spectrometer was interfaced to a custom-built linear time-of-flight (TOF) mass spectrometer for the purpose of examining electrospray-generated plumes. Ionic species that were separated in the ion mobility spectrometer could be selectively determined with the TOF mass spectrometer. Tetraalkylammonium salts, a peptide, and proteins were examined. Their ion mobility spectra typically comprised a few peaks; some of these mobility-resolved species produced characteristic electrospray ions, while others of lower relative mobility did not. The TOF mass spectra of cytochrome c, injected from the ion mobility spectrometer at an indicated temperature of 90 °C or lower, showed signs that were characteristic of protein-solvent clustering.