Adriana Scotti da Silva1, Miriam Falkenberg. 1. Clinical Laboratory Analysis, University Hospital, Federal University of Santa Catarina, Campus Trindade, 88040-970, Florianópolis, Santa Catarina, Brazil.
Abstract
OBJECTIVES: To investigate the analytical interference of drugs in urinary protein and to estimate the lowest interfering concentrations. DESIGN AND METHODS: Drug supplemented urine samples were compared to the control with two reagent strips and the total protein was determined using Pyrogallol Red-Molybdate (PRM). RESULTS: False-positive interferences occurred with Multistix 10 SG for hydroxychloroquine, levofloxacin and ofloxacin. No interference was observed with Combur 10 Test M. Statistically significant false-positive interferences were observed in the PRM assay with all tested drugs, and lowest interfering concentrations were mostly above estimated therapeutic concentrations. The PRM assay "confirmed" the results of the Multistix dipstick, so a real proteinuria could be presumed from the double analytical interference. CONCLUSIONS: This is the first report of analytical interference by quinolone and quinine derivatives in the PRM assay. Special attention to patients using these drugs is needed to minimize errors in the interpretation of urinary protein results.
OBJECTIVES: To investigate the analytical interference of drugs in urinary protein and to estimate the lowest interfering concentrations. DESIGN AND METHODS: Drug supplemented urine samples were compared to the control with two reagent strips and the total protein was determined using Pyrogallol Red-Molybdate (PRM). RESULTS: False-positive interferences occurred with Multistix 10 SG for hydroxychloroquine, levofloxacin and ofloxacin. No interference was observed with Combur 10 Test M. Statistically significant false-positive interferences were observed in the PRM assay with all tested drugs, and lowest interfering concentrations were mostly above estimated therapeutic concentrations. The PRM assay "confirmed" the results of the Multistix dipstick, so a real proteinuria could be presumed from the double analytical interference. CONCLUSIONS: This is the first report of analytical interference by quinolone and quinine derivatives in the PRM assay. Special attention to patients using these drugs is needed to minimize errors in the interpretation of urinary protein results.
Authors: Joris R Delanghe; Marc L De Buyzere; Matthijs Oyaert; Sigurd E Delanghe; Marijn M Speeckaert Journal: Clin Exp Nephrol Date: 2020-02-26 Impact factor: 2.801