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Literature DB >> 21632550

Analysis of intact protein isoforms by mass spectrometry.

Jeremiah D Tipton¹, John C Tran, Adam D Catherman, Dorothy R Ahlf, Kenneth R Durbin, Neil L Kelleher.

Abstract

The diverse proteome of an organism arises from such events as single nucleotide substitutions at the DNA level, different RNA processing, and dynamic enzymatic post-translational modifications. This minireview focuses on the measurement of intact proteins to describe the diversity found in proteomes. The field of biological mass spectrometry has steadily advanced, enabling improvements in the characterization of single proteins to proteins derived from cells or tissues. In this minireview, we discuss the basic technology for "top-down" intact protein analysis. Furthermore, examples of studies involved with the qualitative and quantitative analysis of full-length polypeptides are provided.

Mesh：

Substances：
Protein Isoforms

Year: 2011 PMID： 21632550 PMCID： PMC3138281 DOI： 10.1074/jbc.R111.239442

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

95 in total

1. Novel linear quadrupole ion trap/FT mass spectrometer: performance characterization and use in the comparative analysis of histone H3 post-translational modifications.

Authors: John E P Syka; Jarrod A Marto; Dina L Bai; Stevan Horning; Michael W Senko; Jae C Schwartz; Beatrix Ueberheide; Benjamin Garcia; Scott Busby; Tara Muratore; Jeffrey Shabanowitz; Donald F Hunt
Journal: J Proteome Res Date: 2004 May-Jun Impact factor: 4.466

Analysis of intact protein isoforms by mass spectrometry.

1. Novel linear quadrupole ion trap/FT mass spectrometer: performance characterization and use in the comparative analysis of histone H3 post-translational modifications.

2. High-efficiency nano- and micro-HPLC--high-resolution Orbitrap-MS platform for top-down proteomics.

Review 3. Mass spectrometric imaging for biomedical tissue analysis.

Review 4. Top-down mass spectrometry of integral membrane proteins.

Review 5. Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics.

6. "Proteotyping": population proteomics of human leukocytes using top down mass spectrometry.

Review 7. Proteomics by mass spectrometry: approaches, advances, and applications.

Review 8. Mass spectrometry for structural characterization of therapeutic antibodies.

9. Structural characterization of intact antibodies by high-resolution LTQ Orbitrap mass spectrometry.

Review 10. MALDI imaging mass spectrometry of human tissue: method challenges and clinical perspectives.

Review 1. Proteomic analysis of stem cell differentiation and early development.

2. Comprehensive identification of proteins from MALDI imaging.

3. Eavesdropping on PTM cross-talk through serial enrichment.

Review 4. A mass spectrometry view of stable and transient protein interactions.

5. Experimental Assignment of Disulfide-Bonds in Purified Proteins.

Review 6. Quantitative proteomic analysis of histone modifications.

7. Analysis of intact monoclonal antibody IgG1 by electron transfer dissociation Orbitrap FTMS.

8. The ChroP approach combines ChIP and mass spectrometry to dissect locus-specific proteomic landscapes of chromatin.

Review 9. Regulation, Function, and Detection of Protein Acetylation in Bacteria.

Review 10. Top Down proteomics: facts and perspectives.