Mohsen Khosravi Maharlooei1, Mansooreh Bagheri2, Zhabiz Solhjou1, Behnam Moein Jahromi1, Majid Akrami3, Lili Rohani4, Ahmad Monabati5, Ali Noorafshan6, Gholamhossein Ranjbar Omrani7. 1. Cell and Molecular Medicine Research Group, Student Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Cell and Molecular Medicine Research Group, Student Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: mansooreh_bagheri@yahoo.com. 3. Resident of General Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Laboratory for Stem Cell Research, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. 6. Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran. 7. Endocrine and Metabolism Research Centre, Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
AIMS: Stem cells are a new hope to ameliorate impaired diabetic wound healing. The purpose of this study was to evaluate the effect of adipose tissue derived mesenchymal stem cells (AD-MSCs) on wound healing in a diabetic rat model. METHODS: Twenty-six rats became diabetic by a single intraperitoneal injection of streptozotocin. Six rats served as non-diabetic (non-DM). Diabetic rats were divided into two equal groups randomly; control and treatment. Six weeks later, a full-thickness circular excisional wound was created on the dorsum of each rat. AD-MSCs were injected intra-dermally around the wounds of treatment group. PBS was applied to control and non-DM groups. The wound area was measured every other day. After wound healing completion, full thickness skin samples were taken from the wound sites for evaluation of volume density of collagen fibers, length and volume density of vessels, and numerical density of fibroblasts by stereological methods. RESULTS: AD-MSCs accelerated wound healing rate in diabetic rats, but did not increase length and volume density of the vessels and volume density of the collagen fibers. AD-MSCs decreased the numerical density of fibroblasts. CONCLUSIONS: We concluded that AD-MSCs enhances diabetic wound healing rate probably by other mechanisms rather than enhancing angiogenesis or accumulating collagen fibers.
AIMS: Stem cells are a new hope to ameliorate impaired diabetic wound healing. The purpose of this study was to evaluate the effect of adipose tissue derived mesenchymal stem cells (AD-MSCs) on wound healing in a diabeticrat model. METHODS: Twenty-six rats became diabetic by a single intraperitoneal injection of streptozotocin. Six rats served as non-diabetic (non-DM). Diabeticrats were divided into two equal groups randomly; control and treatment. Six weeks later, a full-thickness circular excisional wound was created on the dorsum of each rat. AD-MSCs were injected intra-dermally around the wounds of treatment group. PBS was applied to control and non-DM groups. The wound area was measured every other day. After wound healing completion, full thickness skin samples were taken from the wound sites for evaluation of volume density of collagen fibers, length and volume density of vessels, and numerical density of fibroblasts by stereological methods. RESULTS: AD-MSCs accelerated wound healing rate in diabeticrats, but did not increase length and volume density of the vessels and volume density of the collagen fibers. AD-MSCs decreased the numerical density of fibroblasts. CONCLUSIONS: We concluded that AD-MSCs enhances diabetic wound healing rate probably by other mechanisms rather than enhancing angiogenesis or accumulating collagen fibers.
Authors: Vivian Capilla-González; Javier López-Beas; Natalia Escacena; Yolanda Aguilera; Antonio de la Cuesta; Rafael Ruiz-Salmerón; Franz Martín; Abdelkrim Hmadcha; Bernat Soria Journal: Mol Ther Date: 2018-08-16 Impact factor: 11.454
Authors: Joanne Li; Yair Pincu; Marina Marjanovic; Andrew J Bower; Eric J Chaney; Tor Jensen; Marni D Boppart; Stephen A Boppart Journal: J Biomed Opt Date: 2016-08-01 Impact factor: 3.170