Haifeng Qiu1, Jiezhi Zhu, Jinjin Yu, Hong Pu, Ruofan Dong. 1. Department of Obstetrics and Gynecology, the Fourth Affiliated Hospital of Suzhou University, Wuxi, China. haifengqiu120@hotmail.com
Abstract
OBJECTIVES: The SLIT2 gene is a novel tumor suppressor gene, whose hypermethylation has been detected in several malignances, including breast cancer, colorectal carcinoma and gliomas. In this study, we assessed the status of SLIT2 and its functions in ovarian cancers and cell lines. METHODS: Methylation-specific PCR was used to investigate the methylated promoter of SLIT2; the functions of SLIT2 in ovarian cancer cells were measured by MTT, colony formation assay and flow cytometry. RESULTS: SLIT2 promoter hypermethylation was detected in 56 of 66 (84.8%) ovarian cancer samples and downregulation of SLIT2 expression in 52 (78.8%). The decreased expression was significantly correlated with SLIT2 promoter hypermethylation (p<0.01). Moreover, reversed expression of SLIT2 suppressed cell growth, migration, colony formation abilities and induced more apoptosis. CONCLUSIONS: These results suggest that SLIT2 is a tumor suppressor in ovarian cancer, and may be a novel target for ovarian cancer treatment.
OBJECTIVES: The SLIT2 gene is a novel tumor suppressor gene, whose hypermethylation has been detected in several malignances, including breast cancer, colorectal carcinoma and gliomas. In this study, we assessed the status of SLIT2 and its functions in ovarian cancers and cell lines. METHODS: Methylation-specific PCR was used to investigate the methylated promoter of SLIT2; the functions of SLIT2 in ovarian cancer cells were measured by MTT, colony formation assay and flow cytometry. RESULTS:SLIT2 promoter hypermethylation was detected in 56 of 66 (84.8%) ovarian cancer samples and downregulation of SLIT2 expression in 52 (78.8%). The decreased expression was significantly correlated with SLIT2 promoter hypermethylation (p<0.01). Moreover, reversed expression of SLIT2 suppressed cell growth, migration, colony formation abilities and induced more apoptosis. CONCLUSIONS: These results suggest that SLIT2 is a tumor suppressor in ovarian cancer, and may be a novel target for ovarian cancer treatment.
Authors: Lui Ng; Ariel K M Chow; Johnny H W Man; Thomas C C Yau; Timothy M H Wan; Deepak N Iyer; Virginia H T Kwan; Ronnie T P Poon; Roberta W C Pang; Wai-Lun Law Journal: BMC Cancer Date: 2018-06-01 Impact factor: 4.430
Authors: Kirti Kaul; Martin Benej; Sanjay Mishra; Dinesh K Ahirwar; Marshleen Yadav; Kristin I Stanford; Naduparambil K Jacob; Nicholas C Denko; Ramesh K Ganju Journal: Front Immunol Date: 2021-10-28 Impact factor: 8.786