Yong Li1, Lu Liang, Xue-Jun Ge. 1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Abstract
PREMISE OF THE STUDY: Microsatellite markers were developed for Pinus koraiensis to characterize its genetic diversity and understand its population structure. • METHODS AND RESULTS: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 20 primer sets were developed in Chinese populations of P. koraiensis. Three of the markers showed polymorphism with two alleles per locus when assessed in a sample of two populations of P. koraiensis from the Changbai Mountain in the Jilin Province of China. Five and three loci were successfully amplified in P. taiwanensis and P. massoniana, respectively. The amplification size of these loci matches those in P. koraiensis. • CONCLUSIONS: These markers may be useful for further investigation of population genetics of P. koraiensis.
PREMISE OF THE STUDY: Microsatellite markers were developed for Pinus koraiensis to characterize its genetic diversity and understand its population structure. • METHODS AND RESULTS: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 20 primer sets were developed in Chinese populations of P. koraiensis. Three of the markers showed polymorphism with two alleles per locus when assessed in a sample of two populations of P. koraiensis from the Changbai Mountain in the Jilin Province of China. Five and three loci were successfully amplified in P. taiwanensis and P. massoniana, respectively. The amplification size of these loci matches those in P. koraiensis. • CONCLUSIONS: These markers may be useful for further investigation of population genetics of P. koraiensis.