PREMISE OF THE STUDY: We present a rapid and inexpensive alternative to DNA isolation for polymerase chain reaction (PCR) amplification from plants. • METHODS AND RESULTS: The method involves direct PCR amplification from material macerated in one buffer, followed by dilution and incubation in a second buffer. We describe the procedure and demonstrate its application for nuclear and plastid DNA amplification across a broad range of vascular plants. • CONCLUSIONS: The method is fast, easy to perform, cost-effective, and consequently ideal for large sample numbers. It represents a considerable simplification of present approaches requiring DNA isolation prior to PCR amplification and will be useful in plant systematics and biotechnology, including applications such as DNA barcoding.
PREMISE OF THE STUDY: We present a rapid and inexpensive alternative to DNA isolation for polymerase chain reaction (PCR) amplification from plants. • METHODS AND RESULTS: The method involves direct PCR amplification from material macerated in one buffer, followed by dilution and incubation in a second buffer. We describe the procedure and demonstrate its application for nuclear and plastid DNA amplification across a broad range of vascular plants. • CONCLUSIONS: The method is fast, easy to perform, cost-effective, and consequently ideal for large sample numbers. It represents a considerable simplification of present approaches requiring DNA isolation prior to PCR amplification and will be useful in plant systematics and biotechnology, including applications such as DNA barcoding.
Authors: Timotheüs Van der Niet; Michael D Pirie; Adam Shuttleworth; Steven D Johnson; Jeremy J Midgley Journal: Ann Bot Date: 2013-09-26 Impact factor: 4.357
Authors: M D Pirie; E G H Oliver; A Mugrabi de Kuppler; B Gehrke; N C Le Maitre; M Kandziora; D U Bellstedt Journal: BMC Evol Biol Date: 2016-09-17 Impact factor: 3.260
Authors: P William Hughes; Allison F Jaworski; Corey S Davis; Susan M Aitken; Andrew M Simons Journal: Appl Plant Sci Date: 2014-04-04 Impact factor: 1.936