BACKGROUND: Pasteurella multocida causes disease in many host species throughout the world. In bovids, it contributes to bovine respiratory disease (BRD) and causes haemorrhagic septicaemia (HS). Previous studies have suggested that BRD-associated P. multocida isolates are of limited diversity. A multilocus sequence typing (MLST) scheme for P. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method used, restricted sample selection or true niche association. Bovine respiratory isolates of P. multocida (n = 133) from the UK, the USA and France, collected between 1984 and 2008 from both healthy and clinically affected animals, were typed using MLST. Isolates of P. multocida from cases of HS, isolates from other host species and data from the MLST database were used as comparison. RESULTS: Bovine respiratory isolates were found to be clonal (I(S)(A) 0.45) with 105/128 belonging to clonal complex 13 (CC13). HS isolates were not related to bovine respiratory isolates. Of the host species studied, the majority had their own unique sequence types (STs), with few STs being shared across host species, although there was some cross over between porcine and bovine respiratory isolates. Avian, ovine and porcine isolates showed greater levels of diversity compared to cattle respiratory isolates, despite more limited geographic origins. CONCLUSIONS: The homogeneity of STs of bovine respiratory P. multocida observed, and the differences between these and P. multocida subpopulations from bovine non-respiratory isolates and non-bovine hosts may indicate niche association.
BACKGROUND:Pasteurella multocida causes disease in many host species throughout the world. In bovids, it contributes to bovinerespiratory disease (BRD) and causes haemorrhagic septicaemia (HS). Previous studies have suggested that BRD-associated P. multocida isolates are of limited diversity. A multilocus sequence typing (MLST) scheme for P. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method used, restricted sample selection or true niche association. Bovine respiratory isolates of P. multocida (n = 133) from the UK, the USA and France, collected between 1984 and 2008 from both healthy and clinically affected animals, were typed using MLST. Isolates of P. multocida from cases of HS, isolates from other host species and data from the MLST database were used as comparison. RESULTS:Bovine respiratory isolates were found to be clonal (I(S)(A) 0.45) with 105/128 belonging to clonal complex 13 (CC13). HS isolates were not related to bovine respiratory isolates. Of the host species studied, the majority had their own unique sequence types (STs), with few STs being shared across host species, although there was some cross over between porcine and bovine respiratory isolates. Avian, ovine and porcine isolates showed greater levels of diversity compared to cattle respiratory isolates, despite more limited geographic origins. CONCLUSIONS: The homogeneity of STs of bovine respiratory P. multocida observed, and the differences between these and P. multocida subpopulations from bovine non-respiratory isolates and non-bovine hosts may indicate niche association.
Authors: Andreas E Zautner; Sahra Herrmann; Jasmin Corso; A Malik Tareen; Thomas Alter; Uwe Gross Journal: Appl Environ Microbiol Date: 2011-01-28 Impact factor: 4.792
Authors: E M Smith; L E Green; G F Medley; H E Bird; L K Fox; Y H Schukken; J V Kruze; A J Bradley; R N Zadoks; C G Dowson Journal: J Clin Microbiol Date: 2005-09 Impact factor: 5.948
Authors: P Mullner; T Shadbolt; J M Collins-Emerson; A C Midwinter; S E F Spencer; J Marshall; P E Carter; D M Campbell; D J Wilson; S Hathaway; R Pirie; N P French Journal: Epidemiol Infect Date: 2010-02-09 Impact factor: 2.451
Authors: Samuel K Sheppard; John F Dallas; Marion MacRae; Noel D McCarthy; E L Sproston; F J Gormley; Norval J C Strachan; Iain D Ogden; Martin C J Maiden; Ken J Forbes Journal: Int J Food Microbiol Date: 2009-02-20 Impact factor: 5.277
Authors: Taya Forde; Roman Biek; Ruth Zadoks; Matthew L Workentine; Jeroen De Buck; Susan Kutz; Tanja Opriessnig; Hannah Trewby; Frank van der Meer; Karin Orsel Journal: BMC Genomics Date: 2016-06-14 Impact factor: 3.969
Authors: Petra Griekspoor; Frances M Colles; Noel D McCarthy; Philip M Hansbro; Chris Ashhurst-Smith; Björn Olsen; Dennis Hasselquist; Martin C J Maiden; Jonas Waldenström Journal: Mol Ecol Date: 2013-01-29 Impact factor: 6.185
Authors: Niloofar Kavousi; Wilhelm Wei Han Eng; Yin Peng Lee; Lian Huat Tan; Ravindran Thuraisingham; Catherine M Yule; Han Ming Gan Journal: Genome Announc Date: 2016-03-03