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Literature DB >> 2160947

Detection of the surface-exposed 18-kilodalton binding protein in Chlamydia trachomatis by immunogold staining.

G J Gray¹, R Kaul, R Sherburne, W M Wenman.

Abstract

Dot-blot analysis of Chlamydia trachomatis elementary bodies (EBs) with monospecific polyclonal antibodies demonstrated that the 18-kilodalton binding protein is surface exposed. Immunoelectron microscopy with whole serovar L2 EBs and ultrathin sections confirmed this finding. In addition, only the extracellular EBs and not the intracellular reticulate bodies were labeled with immunogold.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1990 PMID： 2160947 PMCID： PMC209173 DOI： 10.1128/jb.172.6.3524-3528.1990

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

1. Ultrastructural study of Chlamydia trachomatis surface antigens by immunogold staining with monoclonal antibodies.

Authors: C C Kuo; E Y Chi
Journal: Infect Immun Date: 1987-05 Impact factor: 3.441

2. An immunocolloid method for the electron microscope.

Authors: W P Faulk; G M Taylor
Journal: Immunochemistry Date: 1971-11

3. Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis.

Authors: P Bavoil; A Ohlin; J Schachter
Journal: Infect Immun Date: 1984-05 Impact factor: 3.441

4. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

Authors: J B Olmsted
Journal: J Biol Chem Date: 1981-12-10 Impact factor: 5.157

5. Expression of the Chlamydia trachomatis major outer membrane protein-encoding gene in Escherichia coli: role of the 3' end in mRNA stability.

Authors: R Kaul; M J Duncan; J Guest; W M Wenman
Journal: Gene Date: 1990-03-01 Impact factor: 3.688

6. Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis.

Authors: Y X Zhang; S Stewart; T Joseph; H R Taylor; H D Caldwell
Journal: J Immunol Date: 1987-01-15 Impact factor: 5.422

Detection of the surface-exposed 18-kilodalton binding protein in Chlamydia trachomatis by immunogold staining.

1. Ultrastructural study of Chlamydia trachomatis surface antigens by immunogold staining with monoclonal antibodies.

2. An immunocolloid method for the electron microscope.

3. Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis.

4. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.

5. Expression of the Chlamydia trachomatis major outer membrane protein-encoding gene in Escherichia coli: role of the 3' end in mRNA stability.

6. Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis.

7. Chlamydia trachomatis elementary bodies possess proteins which bind to eucaryotic cell membranes.

8. Identification and properties of chlamydial polypeptides that bind eucaryotic cell surface components.

9. Polypeptide composition of Chlamydia trachomatis.

10. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis.

Review 1. Interaction of chlamydiae and host cells in vitro.

2. Characterization of an immunodominant Giardia lamblia protein antigen related to alpha giardin.