BACKGROUND: Cholangiocarcinoma (CC) is a fatal malignancy, the incidence of which is increasing worldwide, with substantial regional variation. Current diagnostic techniques to distinguish benign from malignant biliary disease are unsatisfactory. Metabolic profiling of bile may help to differentiate benign from malignant disease. No previous studies have compared the metabolic profiles of bile from two geographically and racially distinct groups of CC patients. OBJECTIVES: This study aimed to compare metabolic profiles of bile, using in vitro proton magnetic resonance spectroscopy, from CC patients from Egypt and the UK, and from patients with CC and patients with non-malignant biliary disease. METHODS: A total of 29 bile samples, collected at cholangiography, were analysed using an 11.7-T system. Samples were from eight CC patients in either Egypt (n = 4) or the UK (n = 4) and 21 patients with benign biliary disease (choledocholithiasis [n = 8], sphincter of Oddi dysfunction [n = 8], primary sclerosing cholangitis [n = 5]). RESULTS: Bile phosphatidylcholine (PtC) was significantly reduced in CC patients. Egyptian CC patients had significantly lower biliary PtC levels compared with UK patients. Taurine- and glycine-conjugated bile acids (H-26 and H-25 protons, respectively) were significantly elevated in bile from patients with CC compared with bile from patients with benign diseases (P = 0.013 and P < 0.01, respectively). CONCLUSIONS: Biliary PtC levels potentially differentiate CC from benign biliary disease. Reduced biliary PtC in Egyptian compared with UK patients may reflect underlying carcinogenic mechanisms.
BACKGROUND:Cholangiocarcinoma (CC) is a fatal malignancy, the incidence of which is increasing worldwide, with substantial regional variation. Current diagnostic techniques to distinguish benign from malignant biliary disease are unsatisfactory. Metabolic profiling of bile may help to differentiate benign from malignant disease. No previous studies have compared the metabolic profiles of bile from two geographically and racially distinct groups of CC patients. OBJECTIVES: This study aimed to compare metabolic profiles of bile, using in vitro proton magnetic resonance spectroscopy, from CC patients from Egypt and the UK, and from patients with CC and patients with non-malignant biliary disease. METHODS: A total of 29 bile samples, collected at cholangiography, were analysed using an 11.7-T system. Samples were from eight CC patients in either Egypt (n = 4) or the UK (n = 4) and 21 patients with benign biliary disease (choledocholithiasis [n = 8], sphincter of Oddi dysfunction [n = 8], primary sclerosing cholangitis [n = 5]). RESULTS:Bile phosphatidylcholine (PtC) was significantly reduced in CC patients. Egyptian CC patients had significantly lower biliary PtC levels compared with UK patients. Taurine- and glycine-conjugated bile acids (H-26 and H-25 protons, respectively) were significantly elevated in bile from patients with CC compared with bile from patients with benign diseases (P = 0.013 and P < 0.01, respectively). CONCLUSIONS: Biliary PtC levels potentially differentiate CC from benign biliary disease. Reduced biliary PtC in Egyptian compared with UK patients may reflect underlying carcinogenic mechanisms.
Authors: H Ishikawa; T Nakashima; K Inaba; H Mitsuyoshi; Y Nakajima; Y Sakamoto; T Okanoue; K Kashima; Y Seo Journal: J Lipid Res Date: 1999-10 Impact factor: 5.922
Authors: Shahid A Khan; Simon D Taylor-Robinson; Mireille B Toledano; Angus Beck; Paul Elliott; Howard C Thomas Journal: J Hepatol Date: 2002-12 Impact factor: 25.083
Authors: Annika Gauss; Robert Ehehalt; Wolf-Dieter Lehmann; Gerhard Erben; Karl-Heinz Weiss; Yvonne Schaefer; Petra Kloeters-Plachky; Adolf Stiehl; Wolfgang Stremmel; Peter Sauer; Daniel Nils Gotthardt Journal: World J Gastroenterol Date: 2013-09-07 Impact factor: 5.742
Authors: Munirah Alsaleh; Zoe Leftley; Thomas A Barbera; Larry K Koomson; Abigail Zabron; Mary M E Crossey; Helen L Reeves; Matthew Cramp; Stephen Ryder; Shaun Greer; Martin Prince; Paiboon Sithithaworn; Mohamed Shariff; Narong Khuntikeo; Watcharin Loilome; Puangrat Yongvanit; Yi-Liang Shen; I Jane Cox; Roger Williams; Christopher A Wadsworth; Elaine Holmes; Kathryn Nash; Simon D Taylor-Robinson Journal: J Clin Exp Hepatol Date: 2019-06-15
Authors: Vyacheslav A Petrov; María A Fernández-Peralbo; Rico Derks; Elena M Knyazeva; Nikolay V Merzlikin; Alexey E Sazonov; Oleg A Mayboroda; Irina V Saltykova Journal: Biomed Res Int Date: 2020-07-01 Impact factor: 3.411