BACKGROUND/ PURPOSE: Wound healing is a complex process that involves multiple intercellular and intracellular processes and extracellular interactions. Explanted human skin has been used as a model for the re-epithelialization phase of human wound healing. The currently used standard technique uses a circular punch biopsy tool to make the initial wound. Despite its wide use, the geometry of round wounds makes it difficult to measure them reliably. METHODS: Our group has designed a linear wounding tool, and compared the variability in ex vivo human linear and circular wounds. RESULTS: An F test for differences in variances demonstrated that the linear wounds provided a population of wound size measurements that was 50% less variable than that obtained from a group of matched circular wounds. This reduction in variability would provide substantial advantages for the linear wound technique over the circular wound punch technique, by reducing the sample sizes required for comparative studies of factors that alter healing. CONCLUSION: This linear wounding tool thus provides a method for wounding that is standardized, provides minimal error in wound gap measurements, and is easily reproducible. We demonstrate its utility in an ex vivo model for the controlled investigation of human skin wounds.
BACKGROUND/ PURPOSE: Wound healing is a complex process that involves multiple intercellular and intracellular processes and extracellular interactions. Explanted human skin has been used as a model for the re-epithelialization phase of human wound healing. The currently used standard technique uses a circular punch biopsy tool to make the initial wound. Despite its wide use, the geometry of round wounds makes it difficult to measure them reliably. METHODS: Our group has designed a linear wounding tool, and compared the variability in ex vivo human linear and circular wounds. RESULTS: An F test for differences in variances demonstrated that the linear wounds provided a population of wound size measurements that was 50% less variable than that obtained from a group of matched circular wounds. This reduction in variability would provide substantial advantages for the linear wound technique over the circular wound punch technique, by reducing the sample sizes required for comparative studies of factors that alter healing. CONCLUSION: This linear wounding tool thus provides a method for wounding that is standardized, provides minimal error in wound gap measurements, and is easily reproducible. We demonstrate its utility in an ex vivo model for the controlled investigation of human skin wounds.
Authors: Irena Pastar; Olivera Stojadinovic; Natalie C Yin; Horacio Ramirez; Aron G Nusbaum; Andrew Sawaya; Shailee B Patel; Laiqua Khalid; Rivkah R Isseroff; Marjana Tomic-Canic Journal: Adv Wound Care (New Rochelle) Date: 2014-07-01 Impact factor: 4.730
Authors: George D Glinos; Sebastian H Verne; Adam S Aldahan; Liang Liang; Keyvan Nouri; Sharon Elliot; Marilyn Glassberg; Delia Cabrera DeBuc; Tulay Koru-Sengul; Marjana Tomic-Canic; Irena Pastar Journal: Wound Repair Regen Date: 2018-02-06 Impact factor: 3.617
Authors: Natalia T Meier; Iain S Haslam; David M Pattwell; Guo-You Zhang; Vladimir Emelianov; Roberto Paredes; Sebastian Debus; Matthias Augustin; Wolfgang Funk; Enrique Amaya; Jennifer E Kloepper; Matthew J Hardman; Ralf Paus Journal: PLoS One Date: 2013-09-02 Impact factor: 3.240
Authors: Tomas Komprda; Zbysek Sladek; Zuzana Sevcikova; Veronika Svehlova; Jan Wijacki; Roman Guran; Tomas Do; Zuzana Lackova; Hana Polanska; Lucie Vrlikova; Vendula Popelkova; Petr Michalek; Ondrej Zitka; Marcela Buchtova Journal: Int J Mol Sci Date: 2020-10-24 Impact factor: 5.923