OBJECTIVE: To identify microRNAs (miRNAs) associated with endometrial receptivity. DESIGN: Observational study. SETTING: Medical center. PATIENT(S): Healthy, regularly cycling women undergoing IVF treatment. INTERVENTION(S): Gonadotropin stimulation and endometrial biopsy. MAIN OUTCOME MEASURE(S): Quantification of miRNA expression profiles by deep sequencing. RESULT(S): The miRNA expression profiles in human endometrium on days LH+2 and LH+7 (LH = 0 is the day of the LH surge) in natural cycles as well as on days hCG+4 and hCG+7 (hCG = 0 is the day of hCG injection) in stimulated cycles were determined by deep sequencing. In natural cycles, there were 20 significantly changed miRNAs in human endometrium on LH+7 compared with LH+2. These miRNAs were predicted to target a large set of genes with different functions, including cell cycle, transport, cell adhesion, cell death, and metabolism. In stimulated cycles, 22 miRNAs were significantly dysregulated on hCG+7 in comparison with LH+7, 11 of which exhibited putative estrogen response elements or P response elements in the promoters. Additionally, unsupervised hierarchical clustering analysis demonstrated that the miRNA expression profile on hCG+4 was similar to that on LH+7, suggesting that ovarian stimulation may alter the window of endometrial receptivity. CONCLUSION(S): MiRNAs may be novel biomarkers for human endometrial receptivity and may help optimize the protocol for IVF treatment.
OBJECTIVE: To identify microRNAs (miRNAs) associated with endometrial receptivity. DESIGN: Observational study. SETTING: Medical center. PATIENT(S): Healthy, regularly cycling women undergoing IVF treatment. INTERVENTION(S): Gonadotropin stimulation and endometrial biopsy. MAIN OUTCOME MEASURE(S): Quantification of miRNA expression profiles by deep sequencing. RESULT(S): The miRNA expression profiles in human endometrium on days LH+2 and LH+7 (LH = 0 is the day of the LH surge) in natural cycles as well as on days hCG+4 and hCG+7 (hCG = 0 is the day of hCG injection) in stimulated cycles were determined by deep sequencing. In natural cycles, there were 20 significantly changed miRNAs in human endometrium on LH+7 compared with LH+2. These miRNAs were predicted to target a large set of genes with different functions, including cell cycle, transport, cell adhesion, cell death, and metabolism. In stimulated cycles, 22 miRNAs were significantly dysregulated on hCG+7 in comparison with LH+7, 11 of which exhibited putative estrogen response elements or P response elements in the promoters. Additionally, unsupervised hierarchical clustering analysis demonstrated that the miRNA expression profile on hCG+4 was similar to that on LH+7, suggesting that ovarian stimulation may alter the window of endometrial receptivity. CONCLUSION(S): MiRNAs may be novel biomarkers for human endometrial receptivity and may help optimize the protocol for IVF treatment.
Authors: Signe Altmäe; Francisco J Esteban; Anneli Stavreus-Evers; Carlos Simón; Linda Giudice; Bruce A Lessey; Jose A Horcajadas; Nick S Macklon; Thomas D'Hooghe; Cristina Campoy; Bart C Fauser; Lois A Salamonsen; Andres Salumets Journal: Hum Reprod Update Date: 2013-09-29 Impact factor: 15.610
Authors: Signe Altmäe; Jose A Martinez-Conejero; Francisco J Esteban; Maria Ruiz-Alonso; Anneli Stavreus-Evers; Jose A Horcajadas; Andres Salumets Journal: Reprod Sci Date: 2012-08-17 Impact factor: 3.060
Authors: Hai Yang Hu; Liu He; Kseniya Fominykh; Zheng Yan; Song Guo; Xiaoyu Zhang; Martin S Taylor; Lin Tang; Jie Li; Jianmei Liu; Wen Wang; Haijing Yu; Philipp Khaitovich Journal: Nat Commun Date: 2012 Impact factor: 14.919