| Literature DB >> 21600249 |
Ethlinn V B van Gaal1, Roel van Eijk, Ronald S Oosting, Robbert Jan Kok, Wim E Hennink, Daan J A Crommelin, Enrico Mastrobattista.
Abstract
Screening of new gene delivery candidates regarding transfection efficiency and toxicity is usually performed by reading out transgene expression levels relative to a reference formulation after in vitro transfection. However, over the years and among different laboratories, this screening has been performed in a variety of cell lines, using a variety of conditions and read-out systems, and by comparison to a variety of reference formulations. This makes a direct comparison of results difficult, if not impossible. Reaching a consensus would enable placing new results into context of previous findings and estimate the overall contribution to the improvement of non-viral gene delivery. In this paper we illustrate the sensitivity of transfection outcomes on testing conditions chosen, and propose a screening protocol with the aim of standardization within the field.Entities:
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Year: 2011 PMID： 21600249 DOI： 10.1016/j.jconrel.2011.05.001
Source DB: PubMed Journal: J Control Release ISSN： 0168-3659 Impact factor: 9.776