OBJECTIVE: To evaluate differences in plasma cytokine levels longitudinally in pre-eclamptic and normotensive pregnancies. An increased inflammatory response has long been associated with pre-eclampsia, both early and late in the pre-eclamptic pregnancy. DESIGN: Blood samples were collected longitudinally during pregnancy from a cohort of 1 631 pregnant women. Thirty-two women with pre-eclampsia and 67 normotensive pregnant women were identified from the cohort. SETTING: A Danish regional hospital. SAMPLES: Samples were collected from the 18th week of pregnancy until delivery and divided into the following four gestational intervals: <25th week, 26th-29th week, 30th-35th week and >36th week. METHODS: Simultaneous measurement of all nine cytokines was done using a capture bead system. MAIN OUTCOME MEASURES: Plasma levels of interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, tumor necrosis factor-α, interferon-γ and granulocyte macrophage colony-stimulating factor during pre-eclamptic and normotensive pregnancies. RESULTS: Pre-eclampsia was associated with increased tumor necrosis factor-α between the 26th and 29th week (p=0.0421) and increased IL-6 after the 36th week (p=0.0044). The other cytokines measured were comparable in the two groups. CONCLUSIONS: This large prospective collection of blood samples was undertaken to determine inflammatory status during pre-eclamptic and normotensive pregnancies. Our results support a tendency towards increased inflammation in pre-eclampsia, but the measured cytokines are not eligible for prediction, monitoring or diagnosing pre-eclampsia.
OBJECTIVE: To evaluate differences in plasma cytokine levels longitudinally in pre-eclamptic and normotensive pregnancies. An increased inflammatory response has long been associated with pre-eclampsia, both early and late in the pre-eclamptic pregnancy. DESIGN: Blood samples were collected longitudinally during pregnancy from a cohort of 1 631 pregnant women. Thirty-two women with pre-eclampsia and 67 normotensive pregnant women were identified from the cohort. SETTING: A Danish regional hospital. SAMPLES: Samples were collected from the 18th week of pregnancy until delivery and divided into the following four gestational intervals: <25th week, 26th-29th week, 30th-35th week and >36th week. METHODS: Simultaneous measurement of all nine cytokines was done using a capture bead system. MAIN OUTCOME MEASURES: Plasma levels of interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, tumor necrosis factor-α, interferon-γ and granulocyte macrophage colony-stimulating factor during pre-eclamptic and normotensive pregnancies. RESULTS: Pre-eclampsia was associated with increased tumor necrosis factor-α between the 26th and 29th week (p=0.0421) and increased IL-6 after the 36th week (p=0.0044). The other cytokines measured were comparable in the two groups. CONCLUSIONS: This large prospective collection of blood samples was undertaken to determine inflammatory status during pre-eclamptic and normotensive pregnancies. Our results support a tendency towards increased inflammation in pre-eclampsia, but the measured cytokines are not eligible for prediction, monitoring or diagnosing pre-eclampsia.
Authors: Kelly K Ferguson; John D Meeker; Thomas F McElrath; Bhramar Mukherjee; David E Cantonwine Journal: Am J Obstet Gynecol Date: 2016-12-30 Impact factor: 8.661
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Authors: Brandie D Taylor; Gong Tang; Roberta B Ness; Jørn Olsen; David M Hougaard; Kristin Skogstrand; James M Roberts; Catherine L Haggerty Journal: Pregnancy Hypertens Date: 2015-11-10 Impact factor: 2.899
Authors: Kelly K Ferguson; Thomas F McElrath; Yin-Hsiu Chen; Bhramar Mukherjee; John D Meeker Journal: Am J Reprod Immunol Date: 2014-05-08 Impact factor: 3.886