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Literature DB >> 2158005

Quantitative determination of lymphocyte ACTH1-39.

J A Ferreira¹, M E Carstens, J J Taljaard.

Abstract

A method was developed for quantitative measurement of ACTH1-39, produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence of specific endopeptidases in lymphocytes for processing of the POMC molecule remains doubtful.

Entities: Chemical Disease Gene Species

Mesh：

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Year: 1990 PMID： 2158005 PMCID： PMC7133553 DOI： 10.1016/0143-4179(90)90154-q

Source DB: PubMed Journal: Neuropeptides ISSN： 0143-4179 Impact factor: 3.286

20 in total

1. Effects of purified hypothalamic corticotropin-releasing factor and other substances on the secretion of adrenocorticotropin and beta-endorphin-like immunoactivities in vitro.

Authors: W Vale; C Rivier; L Yang; S Minick; R Guillemin
Journal: Endocrinology Date: 1978-11 Impact factor: 4.736

2. The condensing vacuole of exocrine cells is more acidic than the mature secretory vesicle.

Authors: L Orci; M Ravazzola; R G Anderson
Journal: Nature Date: 1987 Mar 5-11 Impact factor: 49.962

3. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors: A Böyum
Journal: Scand J Clin Lab Invest Suppl Date: 1968

10. Sorting of progeny coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells.

Authors: J Tooze; S A Tooze; S D Fuller
Journal: J Cell Biol Date: 1987-09 Impact factor: 10.539

1 in total

1. Membrane fluidity of platelets and erythrocytes in patients with Alzheimer's disease and the effect of small amounts of aluminium on platelet and erythrocyte membranes.

Authors: S J van Rensburg; M E Carstens; F C Potocnik; A K Aucamp; J J Taljaard; K R Koch
Journal: Neurochem Res Date: 1992-08 Impact factor: 3.996

1 in total

Quantitative determination of lymphocyte ACTH1-39.

1. Effects of purified hypothalamic corticotropin-releasing factor and other substances on the secretion of adrenocorticotropin and beta-endorphin-like immunoactivities in vitro.

2. The condensing vacuole of exocrine cells is more acidic than the mature secretory vesicle.

3. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

4. Protein transport. From organelle to organelle.

5. Isolation of human peripheral blood monocytes: a comparative methodological study.

6. Corticotropin releasing factor induction of leukocyte-derived immunoreactive ACTH and endorphins.

7. Human leukocyte interferon: structural and biological relatedness to adrenocorticotropic hormone and endorphins.

8. Visualization of acidic organelles in intact cells by electron microscopy.

9. Human lymphocyte production of corticotropin and endorphin-like substances: association with leukocyte interferon.

10. Sorting of progeny coronavirus from condensed secretory proteins at the exit from the trans-Golgi network of AtT20 cells.

1. Membrane fluidity of platelets and erythrocytes in patients with Alzheimer's disease and the effect of small amounts of aluminium on platelet and erythrocyte membranes.