Literature DB >> 2157955

Site-directed insertion mutagenesis with cloned fragments in Escherichia coli by P1 phage transduction.

M Izuhara1, R Takata.   

Abstract

A cloned gene with an insertion, which was made by introducing cat, was ligated to the cloning site of the phage lambda gt11. P1 phage grown on cells lysogenized with the recombinant lambda phage could transduce the mutant gene into the original site on the Escherichia coli chromosome.

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Year:  1990        PMID： 2157955     DOI： 10.1007/BF00260506

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  8 in total

1.  Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

Authors:  S C Winans; S J Elledge; J H Krueger; G C Walker
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

2.  Characterization of polynucleotide phosphorylase mutants of Escherichia coli.

Authors:  A M Reiner
Journal:  J Bacteriol       Date:  1969-03       Impact factor: 3.490

3.  Efficient isolation of genes by using antibody probes.

Authors:  R A Young; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

4.  Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.

Authors:  T J Close; R L Rodriguez
Journal:  Gene       Date:  1982-12       Impact factor: 3.688

5.  Cloning and orientation of the gene encoding polynucleotide phosphorylase in Escherichia coli.

Authors:  S Crofton; P P Dennis
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

6.  Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

Authors:  D E Shevell; A M Abou-Zamzam; B Demple; G C Walker
Journal:  J Bacteriol       Date:  1988-07       Impact factor: 3.490

7.  Cloning of the nusA gene of Escherichia coli.

Authors:  T Kurihara; Y Nakamura
Journal:  Mol Gen Genet       Date:  1983

8.  Replacement and amplification of bacterial genes with sequences altered in vitro.

Authors:  N I Gutterson; D E Koshland
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205
  8 in total
  1 in total

1.  Prevalence of RNA polymerase stalling at Escherichia coli promoters after open complex formation.

Authors:  Asma Hatoum; Jeffrey Roberts
Journal:  Mol Microbiol       Date:  2008-04       Impact factor: 3.501
  1 in total

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