Literature DB >> 33112989

"One-step" characterization platform for pathogenic genetics of Staphylococcus aureus.

Yanmei Li1, Yisen Qiu2, Congxiu Ye3, Ling Chen4, Yi Liang5, Teng-Yi Huang6, Li Zhang1, Junyan Liu7.   

Abstract

Staphylococcus aureus (S. aureus) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by S. aureus contaminated food has become a global health problem. S. aureus can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in S. aureus is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in S. aureus, including sea, seb, sec, see, pvl and tsst, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in S. aureus.

Entities:  

Keywords:  Detection; Food borne pathogens; PCR; S. aureus; Toxins

Mesh：

Substances：

Year:  2020        PMID： 33112989     DOI： 10.1007/s00449-020-02449-8

Source DB:  PubMed          Journal:  Bioprocess Biosyst Eng        ISSN： 1615-7591            Impact factor:   3.210


  47 in total

1.  Development and application of a loop-mediated isothermal amplification method on rapid detection Escherichia coli O157 strains from food samples.

Authors:  Xihong Zhao; Yanmei Li; Li Wang; Lijun You; Zhenbo Xu; Lin Li; Xiaowei He; Yao Liu; Jihua Wang; Liansheng Yang
Journal:  Mol Biol Rep       Date:  2009-08-15       Impact factor: 2.316

2.  Reduction and restoration of culturability of beer-stressed and low-temperature-stressed Lactobacillus acetotolerans strain 2011-8.

Authors:  Yang Deng; Junyan Liu; Lin Li; Huijing Fang; Jingxia Tu; Bing Li; Jing Liu; Huiping Li; Zhenbo Xu
Journal:  Int J Food Microbiol       Date:  2015-05-07       Impact factor: 5.277

3.  Virulent and pathogenic features on the Cronobacter sakazakii polymyxin resistant pmr mutant strain s-3.

Authors:  Xuerui Bao; Ling Yang; Lequn Chen; Bing Li; Lin Li; Yanyan Li; Zhenbo Xu
Journal:  Microb Pathog       Date:  2017-07-12       Impact factor: 3.738

4.  Correlation and in vitro mechanism of bactericidal activity on E. coli with whey protein isolate during ultrasonic treatment.

Authors:  Liyan Liu; Ruirui Xu; Lin Li; Bing Li; Xia Zhang; Ximei Zhang; Zhenbo Xu
Journal:  Microb Pathog       Date:  2017-12-24       Impact factor: 3.738

5.  Analysis on pathogenic and virulent characteristics of the Cronobacter sakazakii strain BAA-894 by whole genome sequencing and its demonstration in basic biology science.

Authors:  Xuerui Bao; Ling Yang; Lequn Chen; Bing Li; Lin Li; Yanyan Li; Zhenbo Xu
Journal:  Microb Pathog       Date:  2017-05-22       Impact factor: 3.738

6.  Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products.

Authors:  G Normanno; G La Salandra; A Dambrosio; N C Quaglia; M Corrente; A Parisi; G Santagada; A Firinu; E Crisetti; G V Celano
Journal:  Int J Food Microbiol       Date:  2007-01-16       Impact factor: 5.277

Review 7.  Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management.

Authors:  Steven Y C Tong; Joshua S Davis; Emily Eichenberger; Thomas L Holland; Vance G Fowler
Journal:  Clin Microbiol Rev       Date:  2015-07       Impact factor: 26.132

8.  Integron-bearing methicillin-resistant coagulase-negative staphylococci in South China, 2001-2004.

Authors:  Zhenbo Xu; Lei Shi; M J Alam; Lin Li; Shinji Yamasaki
Journal:  FEMS Microbiol Lett       Date:  2008-01       Impact factor: 2.742

9.  Survival of Salmonella typhimurium and Staphylococcus aureus in eggs cooked by different methods.

Authors:  R C Baker; S Hogarty; W Poon; D V Vadehra
Journal:  Poult Sci       Date:  1983-07       Impact factor: 3.352

10.  Microbial virulence, molecular epidemiology and pathogenic factors of fluoroquinolone-resistant Haemophilus influenzae infections in Guangzhou, China.

Authors:  Dingqiang Chen; Shuxian Wen; Donghua Feng; Ruirui Xu; Junyan Liu; Brian M Peters; Danhong Su; Yongping Lin; Ling Yang; Zhenbo Xu; Mark E Shirtliff
Journal:  Ann Clin Microbiol Antimicrob       Date:  2018-11-23       Impact factor: 3.944
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