| Literature DB >> 2156585 |
O Janssen1, B Heinze, C R Bartram, M Pawlita, T Binder, H Heimpel, K Diehl, D Kabelitz.
Abstract
We have established a new cell line from a patient with centrocytic B cell lymphoma. Highly purified peripheral blood B cells from patient DUL (WBC counts 158,000/microliters) were infected in vitro with Epstein-Barr virus (EBV), and CD20+ B cells were cloned into 96 well culture plates with the aid of a cell sorter autoclone device. As shown by GTG-banding and Southern blot analysis, out-growing EBV-positive clones had the same chromosomal abnormalities and identical monoclonal IgH gene rearrangement as the original EBV-genome-negative leukemic B cell clone. Surface marker analysis with a panel of monoclonal antibodies revealed identical patterns on EBV-negative and -positive clones, with the exception of PCA1 (reactive with plasma cells) which was negative on freshly explanted leukemic B cells but positive on EBV-converted clones.Entities:
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Year: 1990 PMID： 2156585 DOI： 10.1007/BF01720278
Source DB: PubMed Journal: Blut ISSN： 0006-5242