Literature DB >> 21565151

Comparison of three detergent-free protein extraction protocols for white adipose tissue.

Tatjana Sajic1, Gérard Hopfgartner, Ildiko Szanto, Emmanuel Varesio.   

Abstract

A comparative study of three detergent-free protein extraction protocols--a differential centrifugal fractionation, a delipidation protocol based on the Bligh and Dyer method, and the trifluoroethanol addition as cosolvent to an aqueous buffer--was performed on white adipose tissue. The performance of the protocols directly compatible with liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) was evaluated based on the total protein extraction yield and the protein recovery from different functional and cellular compartments. The most suitable method for the extraction of white adipose tissue proteins from a wide range of cellular and structural compartments was the delipidation protocol based on the Bligh and Dyer method.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21565151     DOI： 10.1016/j.ab.2011.04.023

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

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