Literature DB >> 2154965

The effect of the gamma-subunit of the cyclic GMP phosphodiesterase of bovine and frog (Rana catesbiana) retinal rod outer segments on the kinetic parameters of the enzyme.

M M Whalen1, M W Bitensky, D J Takemoto.   

Abstract

Rod-outer-segment cyclic GMP phosphodiesterase (PDE) (subunit composition alpha beta gamma 2) contains catalytic activity in alpha beta. The gamma-subunits are inhibitors. Removal of the gamma-subunits increases Vmax. without affecting the Km. The inhibitory effect of a single gamma-subunit (alpha beta gamma) on the Vmax. of alpha beta is much greater in bovine than in frog (Rana catesbiana) PDE. Bovine PDE in the alpha beta gamma 2 state has a Vmax. that is 2.6 +/- 0.4% of the Vmax. of alpha beta. The removal of one gamma-subunit to give alpha beta gamma results in a Vmax. 5.2 +/- 1% of that for maximal activity. Frog alpha beta gamma 2 has a Vmax. 10.8 +/- 2%, and alpha beta gamma has a Vmax. 50 +/- 18%, of the Vmax. of alpha beta. These data suggest that a single gamma-subunit can inhibit the catalytic activity of active sites on both alpha- and beta-subunits in bovine, but not in frog, rod-outer-segment PDE.

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Year:  1990        PMID： 2154965      PMCID： PMC1133684          DOI： 10.1042/bj2650655

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

1.  Isolation and characterization of cGMP phosphodiesterase from bovine rod outer segments.

Authors:  W Baehr; M J Devlin; M L Applebury
Journal:  J Biol Chem       Date:  1979-11-25       Impact factor: 5.157

2.  Membrane-dependent guanine nucleotide binding and GTPase activities of soluble protein from bovine rod cell outer segments.

Authors:  W Godchaux; W F Zimmerman
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

3.  Purification and properties of the light-activated cyclic nucleotide phosphodiesterase of rod outer segments.

Authors:  N Miki; J M Baraban; J J Keirns; J J Boyce; M W Bitensky
Journal:  J Biol Chem       Date:  1975-08-25       Impact factor: 5.157

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Purification of rod outer segment GTP-binding protein subunits and cGMP phosphodiesterase by single-step column chromatography.

Authors:  A Yamazaki; M Tatsumi; M W Bitensky
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

6.  Multiple cyclic nucleotide phosphodiesterase activities from rat brain.

Authors:  W J Thompson; M M Appleman
Journal:  Biochemistry       Date:  1971-01-19       Impact factor: 3.162

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  cGMP phosphodiesterase of retinal rods is regulated by two inhibitory subunits.

Authors:  P Deterre; J Bigay; F Forquet; M Robert; M Chabre
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

9.  Rod/cone dysplasia in Irish setters. Presence of an altered rhodopsin.

Authors:  J Cunnick; M Rider; L J Takemoto; D J Takemoto
Journal:  Biochem J       Date:  1988-03-01       Impact factor: 3.857

10.  Comparison of the phosphodiesterase inhibitory subunit interactions of frog and bovine rod outer segments.

Authors:  M M Whalen; M W Bitensky
Journal:  Biochem J       Date:  1989-04-01       Impact factor: 3.857
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  8 in total

1.  A quantitative account of the activation steps involved in phototransduction in amphibian photoreceptors.

Authors:  T D Lamb; E N Pugh
Journal:  J Physiol       Date:  1992-04       Impact factor: 5.182

2.  Probing the catalytic sites and activation mechanism of photoreceptor phosphodiesterase using radiolabeled phosphodiesterase inhibitors.

Authors:  Yu-Ting Liu; Suzanne L Matte; Jackie D Corbin; Sharron H Francis; Rick H Cote
Journal:  J Biol Chem       Date:  2009-09-16       Impact factor: 5.157

3.  Regulation of photoreceptor phosphodiesterase catalysis by its non-catalytic cGMP-binding sites.

Authors:  M R D'Amours; R H Cote
Journal:  Biochem J       Date:  1999-06-15       Impact factor: 3.857

4.  Identification of a binding site on retinal transducin alpha for the phosphodiesterase inhibitory gamma subunit.

Authors:  J Cunnick; C Twamley; I Udovichenko; K Gonzalez; D J Takemoto
Journal:  Biochem J       Date:  1994-01-01       Impact factor: 3.857

5.  Binding of the gamma-subunit of retinal rod-outer-segment phosphodiesterase with both transducin and the catalytic subunits of phosphodiesterase.

Authors:  J M Cunnick; D Hurt; B Oppert; K Sakamoto; D J Takemoto
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

6.  Domain mapping of the retinal cyclic GMP phosphodiesterase gamma-subunit. Function of the domains encoded by the three exons of the gamma-subunit gene.

Authors:  D J Takemoto; D Hurt; B Oppert; J Cunnick
Journal:  Biochem J       Date:  1992-02-01       Impact factor: 3.857

7.  It takes two transducins to activate the cGMP-phosphodiesterase 6 in retinal rods.

Authors:  Bilal M Qureshi; Elmar Behrmann; Johannes Schöneberg; Justus Loerke; Jörg Bürger; Thorsten Mielke; Jan Giesebrecht; Frank Noé; Trevor D Lamb; Klaus Peter Hofmann; Christian M T Spahn; Martin Heck
Journal:  Open Biol       Date:  2018-08       Impact factor: 6.411

8.  Rod outer segment structure influences the apparent kinetic parameters of cyclic GMP phosphodiesterase.

Authors:  C L Dumke; V Y Arshavsky; P D Calvert; M D Bownds; E N Pugh
Journal:  J Gen Physiol       Date:  1994-06       Impact factor: 4.086
  8 in total

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