Literature DB >> 2154877

Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera.

M Hofmann1, R Wyler.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushions proved to be the best antigen purification method. Examination of 1024 swine sera showed a high specificity and a greater sensitivity of the ELISA, when compared with indirect immunofluorescence. Reference sera with high antibody titers to PEDV originated from two pigs experimentally infected with PEDV. Three different antigen purification methods and the advantages of the ELISA compared with an immunofluorescence test are discussed.

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Year:  1990        PMID： 2154877      PMCID： PMC7117443          DOI： 10.1016/0378-1135(90)90037-v

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  11 in total

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Journal:  J Immunol Methods       Date:  1988-02-10       Impact factor: 2.303

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Authors:  M Bereiter; J Hasler; H Keller
Journal:  Schweiz Arch Tierheilkd       Date:  1988-05       Impact factor: 0.845

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Authors:  M Hofmann; R Wyler
Journal:  Schweiz Arch Tierheilkd       Date:  1987-08       Impact factor: 0.845

4.  Critical epitopes in transmissible gastroenteritis virus neutralization.

Authors:  G Jiménez; I Correa; M P Melgosa; M J Bullido; L Enjuanes
Journal:  J Virol       Date:  1986-10       Impact factor: 5.103

5.  Principles, problems, and strategies in the use of antigenic mixtures for the enzyme-linked immunosorbent assay.

Authors:  G E Kenny; C L Dunsmoor
Journal:  J Clin Microbiol       Date:  1983-04       Impact factor: 5.948

6.  Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid.

Authors:  L S Sturman; K V Holmes; J Behnke
Journal:  J Virol       Date:  1980-01       Impact factor: 5.103

7.  Experimental infection of pigs with a new porcine enteric coronavirus, CV 777.

Authors:  P Debouck; M Pensaert
Journal:  Am J Vet Res       Date:  1980-02       Impact factor: 1.156

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Journal:  Arch Virol       Date:  1978       Impact factor: 2.574

9.  Coronaviridae.

Authors:  S G Siddell; R Anderson; D Cavanagh; K Fujiwara; H D Klenk; M R Macnaughton; M Pensaert; S A Stohlman; L Sturman; B A van der Zeijst
Journal:  Intervirology       Date:  1983       Impact factor: 1.763

10.  Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.

Authors:  P Callebaut; P Debouck; M Pensaert
Journal:  Vet Microbiol       Date:  1982-09       Impact factor: 3.293
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  20 in total

1.  Antigenic relationships among porcine epidemic diarrhea virus and transmissible gastroenteritis virus strains.

Authors:  Chun-Ming Lin; Xiang Gao; Tomoichiro Oka; Anastasia N Vlasova; Malak A Esseili; Qiuhong Wang; Linda J Saif
Journal:  J Virol       Date:  2015-01-14       Impact factor: 5.103

2.  Evaluation of purified recombinant spike fragments for assessment of the presence of serum neutralizing antibodies against a variant strain of porcine epidemic diarrhea virus.

Authors:  Jianwei Hao; Yun Zhang; Shengkun Fang; Zhifen Wen; Xiangbin Zhang; Chunyi Xue; Yongchang Cao
Journal:  Virol Sin       Date:  2017-07-20       Impact factor: 4.327

3.  Sensitivity and specificity of an enzyme-linked immunosorbent assay for the detection of bovine viral diarrhea virus antibody in cattle.

Authors:  H J Cho; S A Masri; D Deregt; S G Yeo; E J Thomas
Journal:  Can J Vet Res       Date:  1991-01       Impact factor: 1.310

4.  A Computationally Designed Serological Assay for Porcine Epidemic Diarrhea Virus.

Authors:  Yunfeng Song; Pankaj Singh; Eric Nelson; Sheela Ramamoorthy
Journal:  J Clin Microbiol       Date:  2016-05-25       Impact factor: 5.948

Review 5.  Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus.

Authors:  Changhee Lee
Journal:  Virol J       Date:  2015-12-22       Impact factor: 4.099

6.  Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains.

Authors:  Priscilla F Gerber; Davide Lelli; Jianqiang Zhang; Bertel Strandbygaard; Ana Moreno; Antonio Lavazza; Simona Perulli; Anette Bøtner; Loic Comtet; Mickael Roche; Philippe Pourquier; Chong Wang; Tanja Opriessnig
Journal:  Prev Vet Med       Date:  2016-11-08       Impact factor: 2.670

7.  Use of monoclonal antibodies in blocking ELISA detection of transmissible gastroenteritis virus in faeces of piglets.

Authors:  L Rodák; B Smíd; Z Nevoránková; L Valícek; R Smítalová
Journal:  J Vet Med B Infect Dis Vet Public Health       Date:  2005-04

8.  Development of an indirect ELISA, blocking ELISA, fluorescent microsphere immunoassay and fluorescent focus neutralization assay for serologic evaluation of exposure to North American strains of Porcine Epidemic Diarrhea Virus.

Authors:  Faten Okda; Xiaodong Liu; Aaron Singrey; Travis Clement; Julie Nelson; Jane Christopher-Hennings; Eric A Nelson; Steven Lawson
Journal:  BMC Vet Res       Date:  2015-08-01       Impact factor: 2.741

9.  Detection of immunoglobulin (Ig) A antibodies against porcine epidemic diarrhea virus (PEDV) in fecal and serum samples.

Authors:  Priscilla F Gerber; Tanja Opriessnig
Journal:  MethodsX       Date:  2015-10-13

10.  Specific recombinant proteins of porcine epidemic diarrhea virus are immunogenic, revealing their potential use as diagnostic markers.

Authors:  Xi-Mei Lei; Yong-Le Yang; Yong-Qiang He; Lei Peng; Pengwei Zhao; Shu-Ya Xu; Hongwei Cao; Pengfei Fang; Wenying Qiu; Pan Qin; Bin Wang; Yao-Wei Huang
Journal:  Vet Microbiol       Date:  2019-08-10       Impact factor: 3.293
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