PURPOSE: The ability to consistently resolve lamina cribrosa pores in vivo has applications in the study of optic nerve head and retinal disease mechanisms. Repeatability was assessed in imaging laminar pores in normal living eyes with a confocal adaptive optics scanning laser ophthalmoscope (AOSLO). METHODS: Reflectance images (840 nm) of the anterior lamina cribrosa were acquired using the AOSLO in four or more different sessions in two normal rhesus monkey eyes and three normal human eyes. Laminar pore areas, elongations (ratio of major to minor axes of the best-fit ellipse) and nearest neighbor distances were calculated for each session. Measurement repeatability was assessed across sessions. RESULTS: Pore areas ranged from 90 to 4365 μm(2) in monkeys and 154 to 6637 μm(2) in humans. Mean variabilities in measuring pore area and elongation (i.e., mean of the standard deviation of measurements made across sessions for the same pores) were 50 μm(2) (6.1%) and 0.13 (6.7%), respectively, in monkeys and 113 μm(2) (8.3%) and 0.17 (7.7%), respectively, in humans. Mean variabilities in measuring nearest neighbor distances were 1.93 μm (5.2%) in monkeys and 2.79 μm (4.1%) in humans. There were no statistically significant differences in any pore parameters across sessions (ANOVA, P > 0.05). CONCLUSIONS: The anterior lamina cribrosa was consistently imaged in vivo in normal monkey and human eyes. The small intersession variability in normal pore geometry suggests that AOSLO imaging could be used to measure and track changes in laminar pores in vivo during glaucomatous progression.
PURPOSE: The ability to consistently resolve lamina cribrosa pores in vivo has applications in the study of optic nerve head and retinal disease mechanisms. Repeatability was assessed in imaging laminar pores in normal living eyes with a confocal adaptive optics scanning laser ophthalmoscope (AOSLO). METHODS: Reflectance images (840 nm) of the anterior lamina cribrosa were acquired using the AOSLO in four or more different sessions in two normal rhesus monkey eyes and three normal human eyes. Laminar pore areas, elongations (ratio of major to minor axes of the best-fit ellipse) and nearest neighbor distances were calculated for each session. Measurement repeatability was assessed across sessions. RESULTS: Pore areas ranged from 90 to 4365 μm(2) in monkeys and 154 to 6637 μm(2) in humans. Mean variabilities in measuring pore area and elongation (i.e., mean of the standard deviation of measurements made across sessions for the same pores) were 50 μm(2) (6.1%) and 0.13 (6.7%), respectively, in monkeys and 113 μm(2) (8.3%) and 0.17 (7.7%), respectively, in humans. Mean variabilities in measuring nearest neighbor distances were 1.93 μm (5.2%) in monkeys and 2.79 μm (4.1%) in humans. There were no statistically significant differences in any pore parameters across sessions (ANOVA, P > 0.05). CONCLUSIONS: The anterior lamina cribrosa was consistently imaged in vivo in normal monkey and human eyes. The small intersession variability in normal pore geometry suggests that AOSLO imaging could be used to measure and track changes in laminar pores in vivo during glaucomatous progression.
Authors: Ian A Sigal; Richard A Bilonick; Larry Kagemann; Gadi Wollstein; Hiroshi Ishikawa; Joel S Schuman; Jonathan L Grimm Journal: Invest Ophthalmol Vis Sci Date: 2012-05-04 Impact factor: 4.799
Authors: Zach Nadler; Bo Wang; Gadi Wollstein; Jessica E Nevins; Hiroshi Ishikawa; Larry Kagemann; Ian A Sigal; R Daniel Ferguson; Daniel X Hammer; Ireneusz Grulkowski; Jonathan J Liu; Martin F Kraus; Chen D Lu; Joachim Hornegger; James G Fujimoto; Joel S Schuman Journal: Biomed Opt Express Date: 2013-10-24 Impact factor: 3.732
Authors: Monica F Chen; Toco Y P Chui; Paula Alhadeff; Richard B Rosen; Robert Ritch; Alfredo Dubra; Donald C Hood Journal: Invest Ophthalmol Vis Sci Date: 2015-01-08 Impact factor: 4.799
Authors: Howard Lockwood; Juan Reynaud; Stuart Gardiner; Jonathan Grimm; Vincent Libertiaux; J Crawford Downs; Hongli Yang; Claude F Burgoyne Journal: Invest Ophthalmol Vis Sci Date: 2015-02-03 Impact factor: 4.799