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Literature DB >> 2154165

DNA topoisomerase I from Alcaligenes eutrophus H16.

Abstract

Molecular and functional properties of DNA topoisomerase I isolated from a hydrogen-oxidizing bacterium, Alcaligenes eutrophus H16, were investigated. Under native conditions the enzyme forms a monomer with a relative molar mass of 98,500. A rod-like shape of the molecule was derived from the calculated frictional coefficient. The isoelectric point of the enzyme was determined to be in the range of 7.6-8.0. The enzyme activity is strictly Mg2+ dependent with an optimum at 3 mM Mg2+. The pH optimum ranges within 7.5-9.0. A. eutrophus DNA topoisomerase I activity is inhibited by M13 ssDNA, high ionic strength, polyamines, heparin and by a number of intercalating drugs.

Entities: Chemical Species

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Year: 1990 PMID： 2154165 DOI： 10.1007/BF00247810

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

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References

28 in total

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Authors: C W Tabor; L G Dobbs
Journal: J Biol Chem Date: 1970-04-25 Impact factor: 5.157

DNA topoisomerase I from Alcaligenes eutrophus H16.

1. [A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies].

2. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Review 3. Recent studies of DNA topoisomerases.

4. DNA modifying enzymes of Agrobacterium tumefaciens: effect of DNA topoisomerase, restriction endonuclease, and unique DNA endonuclease on plasmid and plant DNA.

5. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

6. Metabolism of 1,4-diaminobutane and spermidine in Escherichia coli: the effects of low temperature during storage and harvesting of cultures.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 8. Biology of bacterial deoxyribonucleic acid topoisomerases.

9. Mechanism of inhibition of mammalian DNA topoisomerase I by heparin.

10. Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16.