Literature DB >> 2153958

Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: [Fe-S] cluster-driven protein rearrangement.

A E Martín1, B K Burgess, C D Stout, V L Cash, D R Dean, G M Jensen, P J Stephens.   

Abstract

Azotobacter vinelandii ferredoxin I is a small protein that contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. Recently the x-ray crystal structure has been redetermined and the fdxA gene, which encodes the protein, has been cloned and sequenced. Here we report the site-directed mutation of Cys-20, which is a ligand of the [4Fe-4S] cluster in the native protein, to alanine and the characterization of the protein product by x-ray crystallographic and spectroscopic methods. The data show that the mutant protein again contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. The new [4Fe-4S] cluster obtains its fourth ligand from Cys-24, a free cysteine in the native structure. The formation of this [4Fe-4S] cluster drives rearrangement of the protein structure.

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Year:  1990        PMID： 2153958      PMCID： PMC53312          DOI： 10.1073/pnas.87.2.598

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Crystallographic structure refinement of Chromatium high potential iron protein at two Angstroms resolution.

Authors:  S T Freer; R A Alden; C W Carter; J Kraut
Journal:  J Biol Chem       Date:  1975-01-10       Impact factor: 5.157

2.  Azotobacter vinelandii ferredoxin I: cloning, sequencing, and mutant analysis.

Authors:  T V Morgan; D J Lundell; B K Burgess
Journal:  J Biol Chem       Date:  1988-01-25       Impact factor: 5.157

3.  Pseudomonas stutzeri ferredoxin: close similarity to Azotobacter vinelandii and Pseudomonas ovalis ferredoxins.

Authors:  K Saeki; S Wakabayashi; W G Zumft; H Matsubara
Journal:  J Biochem       Date:  1988-08       Impact factor: 3.387

Review 4.  Structure, function and evolution of bacterial ferredoxins.

Authors:  M Bruschi; F Guerlesquin
Journal:  FEMS Microbiol Rev       Date:  1988 Apr-Jun       Impact factor: 16.408

5.  Two biologically active ferredoxins from the aerobic nitrogen-fixing bacteriu, Azotobacter vinelandii.

Authors:  D C Yoch; D I Arnon
Journal:  J Biol Chem       Date:  1972-07-25       Impact factor: 5.157

6.  Low-resolution electron-density and anomalous-scattering-density maps of Chromatium high-potential iron protein.

Authors:  G Strahs; J Kraut
Journal:  J Mol Biol       Date:  1968-08-14       Impact factor: 5.469

7.  High and low reduction potential 4Fe-4S clusters in Azotobacter vinelandii (4Fe-4S) 2ferredoxin I. Influence of the polypeptide on the reduction potentials.

Authors:  W V Sweeney; J C Rabinowitz; D C Yoch
Journal:  J Biol Chem       Date:  1975-10-10       Impact factor: 5.157

8.  7-Iron ferredoxin revisited.

Authors:  C D Stout
Journal:  J Biol Chem       Date:  1988-07-05       Impact factor: 5.157

9.  Iron-molybdenum cofactor biosynthesis in Azotobacter vinelandii requires the iron protein of nitrogenase.

Authors:  A C Robinson; D R Dean; B K Burgess
Journal:  J Biol Chem       Date:  1987-10-15       Impact factor: 5.157

10.  Refinement of the 7 Fe ferredoxin from Azotobacter vinelandii at 1.9 A resolution.

Authors:  C D Stout
Journal:  J Mol Biol       Date:  1989-02-05       Impact factor: 5.469
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  14 in total

1.  Asp1 from Schizosaccharomyces pombe binds a [2Fe-2S](2+) cluster which inhibits inositol pyrophosphate 1-phosphatase activity.

Authors:  Huanchen Wang; Vasudha S Nair; Ashley A Holland; Samanta Capolicchio; Henning J Jessen; Michael K Johnson; Stephen B Shears
Journal:  Biochemistry       Date:  2015-10-09       Impact factor: 3.162

2.  Role of aromatic residues in stabilization of the [Fe4S4] cluster in high-potential iron proteins (HiPIPs): physical characterization and stability studies of Tyr-19 mutants of Chromatium vinosum HiPIP.

Authors:  A Agarwal; D Li; J A Cowan
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-10       Impact factor: 11.205

3.  In Azotobacter vinelandii hydrogenase, substitution of serine for the cysteine residues at positions 62, 65, 294, and 297 in the small (HoxK) subunit affects H2 oxidation [corrected].

Authors:  L A Sayavedra-Soto; D J Arp
Journal:  J Bacteriol       Date:  1993-06       Impact factor: 3.490

4.  The genes encoding the delta subunits of dinitrogenases 2 and 3 are required for mo-independent diazotrophic growth by Azotobacter vinelandii.

Authors:  S I Waugh; D M Paulsen; P V Mylona; R H Maynard; R Premakumar; P E Bishop
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490

Review 5.  Electron Transfer in Nitrogenase.

Authors:  Hannah L Rutledge; F Akif Tezcan
Journal:  Chem Rev       Date:  2020-01-30       Impact factor: 60.622

6.  The hydrogenase cytochrome b heme ligands of Azotobacter vinelandii are required for full H(2) oxidation capability.

Authors:  L Meek; D J Arp
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

7.  Site-directed mutagenesis of Azotobacter vinelandii ferredoxin I: cysteine ligation of the [4Fe-4S] cluster with protein rearrangement is preferred over serine ligation.

Authors:  B Shen; D R Jollie; T C Diller; C D Stout; P J Stephens; B K Burgess
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

8.  Construction of a bisaquo heme enzyme and binding by exogenous ligands.

Authors:  D E McRee; G M Jensen; M M Fitzgerald; H A Siegel; D B Goodin
Journal:  Proc Natl Acad Sci U S A       Date:  1994-12-20       Impact factor: 11.205

9.  Insight into the protein and solvent contributions to the reduction potentials of [4Fe-4S]2+/+ clusters: crystal structures of the Allochromatium vinosum ferredoxin variants C57A and V13G and the homologous Escherichia coli ferredoxin.

Authors:  Emmanuel Saridakis; Petros Giastas; Georgios Efthymiou; Vladimiros Thoma; Jean-Marc Moulis; Panayotis Kyritsis; Irene M Mavridis
Journal:  J Biol Inorg Chem       Date:  2009-03-17       Impact factor: 3.358

10.  Altered nitrogenase MoFe proteins from Azotobacter vinelandii. Analysis of MoFe proteins having amino acid substitutions for the conserved cysteine residues within the beta-subunit.

Authors:  H D May; D R Dean; W E Newton
Journal:  Biochem J       Date:  1991-07-15       Impact factor: 3.857
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