OBJECTIVE: Specific acquired HRAS mutations have been found to predominate in bladder cancer, and HRAS T81C polymorphism has been determined to contribute the risk of various cancers, including bladder cancer. MATERIALS AND METHODS: We screened the exon 1and 2 of HRAS and frequently detected polymorphism at nucleotide 81T to C (exon 1). A case-control study was conducted using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to test the genotype distribution of 140 bladder cancer patients in comparison with 160 cancer-free controls from a Kashmiri population. RESULTS: In HRAS T81C SNP, frequencies of TT, TC, and CC genotypes among controls were 84.4%, 15.6%, and 0.0%, while in cases allele frequencies were 64.3%, 30%, and 5.7%, respectively. A significant differences was observed between the control and cases with odds ratio (OR) = 3.0 and 95% confidence interval (CI) = 1.74-5.20 (P = 0.000). Interestingly, combined TC and CC genotype abundantly presented in high grade (OR = 5.4 and 95% CI = 2.8-10.2; P < 0.00) and in advanced tumors (OR = 3.3, 95% CI = 1.71-6.30; P < 0.05). A significant association of the variant allele (TC+CC) was found with male subjects (≥50) and ever smokers (P = 0.001). CONCLUSION: It is evident from our study that HRAS T81C SNP moderately increases bladder cancer risk, and rare allele is a predictive marker of advanced bladder tumors.
OBJECTIVE: Specific acquired HRAS mutations have been found to predominate in bladder cancer, and HRAST81C polymorphism has been determined to contribute the risk of various cancers, including bladder cancer. MATERIALS AND METHODS: We screened the exon 1and 2 of HRAS and frequently detected polymorphism at nucleotide 81T to C (exon 1). A case-control study was conducted using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to test the genotype distribution of 140 bladder cancerpatients in comparison with 160 cancer-free controls from a Kashmiri population. RESULTS: In HRAST81C SNP, frequencies of TT, TC, and CC genotypes among controls were 84.4%, 15.6%, and 0.0%, while in cases allele frequencies were 64.3%, 30%, and 5.7%, respectively. A significant differences was observed between the control and cases with odds ratio (OR) = 3.0 and 95% confidence interval (CI) = 1.74-5.20 (P = 0.000). Interestingly, combined TC and CC genotype abundantly presented in high grade (OR = 5.4 and 95% CI = 2.8-10.2; P < 0.00) and in advanced tumors (OR = 3.3, 95% CI = 1.71-6.30; P < 0.05). A significant association of the variant allele (TC+CC) was found with male subjects (≥50) and ever smokers (P = 0.001). CONCLUSION: It is evident from our study that HRAST81C SNP moderately increases bladder cancer risk, and rare allele is a predictive marker of advanced bladder tumors.
Authors: Mosin S Khan; Arshad A Pandith; Mahboob Ul Hussain; Mohammad Iqbal; Nighat P Khan; Khurshid A Wani; Shariq R Masoodi; Syed Mudassar Journal: Tumour Biol Date: 2012-11-13
Authors: Sara Tomei; Sharon Adams; Lorenzo Uccellini; Davide Bedognetti; Valeria De Giorgi; Narnygerel Erdenebileg; Maria Libera Ascierto; Jennifer Reinboth; Qiuzhen Liu; Generoso Bevilacqua; Ena Wang; Chiara Mazzanti; Francesco M Marincola Journal: Med Oncol Date: 2012-05-22 Impact factor: 3.064
Authors: Nadezda Lipunova; Anke Wesselius; Kar K Cheng; Frederik J van Schooten; Jean-Baptiste Cazier; Richard T Bryan; Maurice P Zeegers Journal: Biomark Cancer Date: 2019-12-30
Authors: Magdalena Traczyk; Edyta Borkowska; Marek Rożniecki; Rafał Purpurowicz; Adam Jędrzejczyk; Piotr Marks; Michał Pietrusiński; Zbigniew Jabłonowski; Marek Sosnowski; Bogdan Kałużewski Journal: Cent European J Urol Date: 2012-06-12