Literature DB >> 21504045

A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.

Go Shioi1, Hiroshi Kiyonari, Takaya Abe, Kazuki Nakao, Toshihiko Fujimori, Chuan-Wei Jang, Cheng-Chiu Huang, Haruhiko Akiyama, Richard R Behringer, Shinichi Aizawa.   

Abstract

Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.
Copyright © 2011 Wiley-Liss, Inc.

Entities:  

Mesh：

Year:  2011        PMID： 21504045      PMCID： PMC3466090          DOI： 10.1002/dvg.20758

Source DB:  PubMed          Journal:  Genesis        ISSN： 1526-954X            Impact factor:   2.487


  26 in total

Review 1.  Cre recombinase: the universal reagent for genome tailoring.

Authors:  A Nagy
Journal:  Genesis       Date:  2000-02       Impact factor: 2.487

2.  Photomodulatable fluorescent proteins for imaging cell dynamics and cell fate.

Authors:  Sonja Nowotschin; Anna-Katerina Hadjantonakis
Journal:  Organogenesis       Date:  2009-10       Impact factor: 2.500

Review 3.  Development of 2A peptide-based strategies in the design of multicistronic vectors.

Authors:  Andrea L Szymczak; Dario A A Vignali
Journal:  Expert Opin Biol Ther       Date:  2005-05       Impact factor: 4.388

4.  Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors.

Authors:  Haruhiko Akiyama; Jung-Eun Kim; Kazuhisa Nakashima; Gener Balmes; Naomi Iwai; Jian Min Deng; Zhaoping Zhang; James F Martin; Richard R Behringer; Takashi Nakamura; Benoit de Crombrugghe
Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-03       Impact factor: 11.205

5.  Generalized lacZ expression with the ROSA26 Cre reporter strain.

Authors:  P Soriano
Journal:  Nat Genet       Date:  1999-01       Impact factor: 38.330

6.  Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells.

Authors:  B P Zambrowicz; A Imamoto; S Fiering; L A Herzenberg; W G Kerr; P Soriano
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

7.  Tissue-inherent fate of GPI revealed by GPI-anchored GFP transgenesis.

Authors:  G Kondoh; X H Gao; Y Nakano; H Koike; S Yamada; M Okabe; J Takeda
Journal:  FEBS Lett       Date:  1999-09-24       Impact factor: 4.124

8.  Dual transgene strategy for live visualization of chromatin and plasma membrane dynamics in murine embryonic stem cells and embryonic tissues.

Authors:  Sonja Nowotschin; Guy S Eakin; Anna-Katerina Hadjantonakis
Journal:  Genesis       Date:  2009-05       Impact factor: 2.487

9.  Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase.

Authors:  P S Danielian; D Muccino; D H Rowitch; S K Michael; A P McMahon
Journal:  Curr Biol       Date:  1998-12-03       Impact factor: 10.834

10.  High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi.

Authors:  Peter Hohenstein; Joan Slight; Derya Deniz Ozdemir; Sally F Burn; Rachel Berry; Nicholas D Hastie
Journal:  Pathogenetics       Date:  2008-11-03
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  21 in total

1.  The development and plasticity of alveolar type 1 cells.

Authors:  Jun Yang; Belinda J Hernandez; Denise Martinez Alanis; Odemaris Narvaez del Pilar; Lisandra Vila-Ellis; Haruhiko Akiyama; Scott E Evans; Edwin J Ostrin; Jichao Chen
Journal:  Development       Date:  2015-11-19       Impact factor: 6.868

2.  A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors.

Authors:  Carrie B Wiese; Nicole Fleming; Dennis P Buehler; E Michelle Southard-Smith
Journal:  Genesis       Date:  2013-10-21       Impact factor: 2.487

Review 3.  Imaging morphogenesis.

Authors:  Donald M Bell
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2017-05-19       Impact factor: 6.237

4.  Tissue-Specific Regulation of Oncogene Expression Using Cre-Inducible ROSA26 Knock-In Transgenic Mice.

Authors:  Brandi L Carofino; Monica J Justice
Journal:  Curr Protoc Mouse Biol       Date:  2015-06-01

5.  Live imaging fluorescent proteins in early mouse embryos.

Authors:  Panagiotis Xenopoulos; Sonja Nowotschin; Anna-Katerina Hadjantonakis
Journal:  Methods Enzymol       Date:  2012       Impact factor: 1.600

6.  Sox9 overexpression in uterine epithelia induces endometrial gland hyperplasia.

Authors:  Gabriel Gonzalez; Shyamin Mehra; Ying Wang; Haruhiko Akiyama; Richard R Behringer
Journal:  Differentiation       Date:  2016-06-01       Impact factor: 3.880

7.  A dual-color genetically engineered mouse model for multispectral imaging of the pancreatic microenvironment.

Authors:  Cynthia S Snyder; Austin R Harrington; Sharmeela Kaushal; Evangeline Mose; Andrew M Lowy; Robert M Hoffman; Michael Bouvet
Journal:  Pancreas       Date:  2013-08       Impact factor: 3.327

8.  Expression of a membrane-targeted fluorescent reporter disrupts auditory hair cell mechanoelectrical transduction and causes profound deafness.

Authors:  Angela Ballesteros; Tracy S Fitzgerald; Kenton J Swartz
Journal:  Hear Res       Date:  2021-02-18       Impact factor: 3.208

9.  Cell influx and contractile actomyosin force drive mammary bud growth and invagination.

Authors:  Ewelina Trela; Qiang Lan; Satu-Marja Myllymäki; Clémentine Villeneuve; Riitta Lindström; Vinod Kumar; Sara A Wickström; Marja L Mikkola
Journal:  J Cell Biol       Date:  2021-05-27       Impact factor: 10.539

10.  R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.

Authors:  You-Tzung Chen; Ming-Shian Tsai; Tsung-Lin Yang; Amy Tsu Ku; Ke-Han Huang; Cheng-Yen Huang; Fu-Ju Chou; Hsiang-Hsuan Fan; Jin-Bon Hong; Shuo-Ting Yen; Wei-Le Wang; Chang-Ching Lin; Yu-Chen Hsu; Kang-Yi Su; I-Chang Su; Chuan-Wei Jang; Richard R Behringer; Rebecca Favaro; Silvia K Nicolis; Chung-Liang Chien; Shu-Wha Lin; I-Shing Yu
Journal:  PLoS One       Date:  2012-09-25       Impact factor: 3.240
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