| Literature DB >> 10570928 |
G Kondoh1, X H Gao, Y Nakano, H Koike, S Yamada, M Okabe, J Takeda.
Abstract
To clarify the fate of glycosylphosphatidylinositol (GPI) in mammals, we developed GPI-anchored enhanced green fluorescent protein (EGFP-GPI) and transgenic mice carrying this fusion construct. When it was introduced to culture cells, the EGFP-GPI protein was correctly sorted to plasma membranes and microsomes depending on GPI biosynthesis. Transgenic mice carrying EGFP-GPI were found to show a broad transgene expression. Histologically, a prominent polarized localization of EGFP-GPI protein was observed in various epithelia, the nervous system and liver and secreted from some exocrine glands, as well as non-polarized presence in non-epithelial tissues, demonstrating a tissue-inherent manner of GPI sorting.Entities:
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Year: 1999 PMID： 10570928 DOI： 10.1016/s0014-5793(99)01172-2
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124