Literature DB >> 21498751

Microarray-based evaluation of whole-community genome DNA amplification methods.

Jian Wang1, Joy D Van Nostrand, Liyou Wu, Zhili He, Guanghe Li, Jizhong Zhou.   

Abstract

Three whole-community genome amplification methods, Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias than Bst. For community DNA, REPLI-g showed the least bias and highest number of genes, while Bst had the highest success rate and was suitable for low-quality DNA.

Mesh：

Year:  2011        PMID： 21498751      PMCID： PMC3131669          DOI： 10.1128/AEM.01834-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  29 in total

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  11 in total

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