BACKGROUND/AIMS: Few reports have described the association between mutations in the entire X gene of the hepatitis B virus (HBV) and the clinical status of HBV-infected patients. We studied the association between HBV X gene mutations and the disease status of patients infected with HBV genotype C. METHODS: Mutations in the HBV X genes of 194 patients were determined by direct sequencing. The subject population consisted of patients with chronic hepatitis (n=60), liver cirrhosis (n=65), and hepatocellular carcinoma (HCC) (n=69). The sequencing results of these 3 groups were compared. RESULTS: Each of the mutations G1386M, C1485T, C1653T, T1753V, A1762T, and G1764A was significantly associated with the patient's clinical status. The T1753V (p<0.001) and A1762T/G1764A (p<0.001) mutations were found more frequently in Hepatitis B e antigen (HBeAg)-negative than in HBeAg-positive patients. Specific X gene mutations (G1386M, C1653T, and A1762T/G1764A) were more prevalent in patients with liver cirrhosis and HCC than in chronic hepatitis patients (p<0.005 for all). In addition, the T1753V (p<0.001) and C1485T (p<0.001) mutations were significantly more prevalent in HCC patients than in chronic hepatitis patients. Only the prevalence of the T1753V mutation increased as the HBV infection progressed from liver cirrhosis to HCC (p=0.023). CONCLUSIONS: Our findings show a difference in the pattern of X gene mutations that were associated with the clinical status of patients with chronic HBV infection.
BACKGROUND/AIMS: Few reports have described the association between mutations in the entire X gene of the hepatitis B virus (HBV) and the clinical status of HBV-infectedpatients. We studied the association between HBV X gene mutations and the disease status of patients infected with HBV genotype C. METHODS: Mutations in the HBV X genes of 194 patients were determined by direct sequencing. The subject population consisted of patients with chronic hepatitis (n=60), liver cirrhosis (n=65), and hepatocellular carcinoma (HCC) (n=69). The sequencing results of these 3 groups were compared. RESULTS: Each of the mutations G1386M, C1485T, C1653T, T1753V, A1762T, and G1764A was significantly associated with the patient's clinical status. The T1753V (p<0.001) and A1762T/G1764A (p<0.001) mutations were found more frequently in Hepatitis B e antigen (HBeAg)-negative than in HBeAg-positive patients. Specific X gene mutations (G1386M, C1653T, and A1762T/G1764A) were more prevalent in patients with liver cirrhosis and HCC than in chronic hepatitispatients (p<0.005 for all). In addition, the T1753V (p<0.001) and C1485T (p<0.001) mutations were significantly more prevalent in HCC patients than in chronic hepatitispatients. Only the prevalence of the T1753V mutation increased as the HBV infection progressed from liver cirrhosis to HCC (p=0.023). CONCLUSIONS: Our findings show a difference in the pattern of X gene mutations that were associated with the clinical status of patients with chronic HBV infection.
Entities:
Keywords:
Cirrhosis; Hepatitis B virus; Hepatocellular carcinoma; Liver disease; X mutation
Authors: Qingming Dong; Henry L Y Chan; Zheng Liu; Denise P C Chan; Bao Zhang; Yangchao Chen; Hsiang-Fu Kung; Joseph J Y Sung; Ming-Liang He Journal: Biochem Biophys Res Commun Date: 2008-08-08 Impact factor: 3.575
Authors: Ja Kyung Kim; Hye Young Chang; Jung Min Lee; Oidov Baatarkhuu; Young Joon Yoon; Jun Yong Park; Do Young Kim; Kwang-Hyub Han; Chae Yoon Chon; Sang Hoon Ahn Journal: J Med Virol Date: 2009-06 Impact factor: 2.327
Authors: Joseph J Y Sung; Stephen K W Tsui; Chi-Hang Tse; Eddie Y T Ng; Kwong-Sak Leung; Kin-Hong Lee; Tony S K Mok; Angeline Bartholomeusz; Thomas C C Au; Kelvin K F Tsoi; Stephen Locarnini; Henry L Y Chan Journal: J Virol Date: 2008-01-23 Impact factor: 5.103