| Literature DB >> 21460824 |
Jian-Quan Ni1, Rui Zhou, Benjamin Czech, Lu-Ping Liu, Laura Holderbaum, Donghui Yang-Zhou, Hye-Seok Shim, Rong Tao, Dominik Handler, Phillip Karpowicz, Richard Binari, Matthew Booker, Julius Brennecke, Lizabeth A Perkins, Gregory J Hannon, Norbert Perrimon.
Abstract
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.Entities:
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Year: 2011 PMID： 21460824 PMCID： PMC3489273 DOI： 10.1038/nmeth.1592
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547