Spectroscopic studies on TiO(2) enhanced binding of Hypocrellin B with DNA.

The binding of Hypocrellin B-TiO(2) chelate with DNA has been studied by using absorption, steady state fluorescence, cyclic voltammetry, time resolved fluorescence and laser flash photolysis measurements. The experimental results show that the presence of TiO(2) nanoparticles increases the binding of Hypocrellin B with DNA. The groove binding mode is confirmed by spectroscopic and docking studies. Laser flash photolysis studies confirm the presence of electrons in the conduction band of TiO(2) which will produce active oxygen species and results in damage of DNA indicating the potential application of Hypocrellin B-TiO(2) chelate in the field of photodynamic therapy (PDT).