BACKGROUND/AIMS: The CXCR4/SDF-1 axis regulates the trafficking of various cell types. In the study we investigated whether adipose-derived stem cells can promote gastric cancer cell growth and migration through SDF-1/CXCR4 axis. METHODOLOGY: The expression of CXCR4 in gastric cancer cell line MGC-803 and SDF-1 in Adipose-derived stem cells was detected by using PCR method. Furthermore, cell proliferation, migration and invasion assays were examined and inhibitors of CXCR4 were used to determine the effect on cell migration. RESULTS: The cells isolated from subcutaneous adipose tissue show characteristics of stem cells. Gastric cancer cells express CXCR4 and Adipose-derived stem cells express SDF-1. Additionally, the tumor cells showed vigorous proliferative and migratory responses to ADSCs. AMD3100 can effectively reduce the migration of gastric cancer cells. CONCLUSIONS: Adipose-derived stem cells can promote gastric cancer cell growth, migration and invasion through SDF-1/CXCR4 axis. CXCR4 can be used in the treatment of gastric cancer as a potential therapeutic target.
BACKGROUND/AIMS: The CXCR4/SDF-1 axis regulates the trafficking of various cell types. In the study we investigated whether adipose-derived stem cells can promote gastric cancer cell growth and migration through SDF-1/CXCR4 axis. METHODOLOGY: The expression of CXCR4 in gastric cancer cell line MGC-803 and SDF-1 in Adipose-derived stem cells was detected by using PCR method. Furthermore, cell proliferation, migration and invasion assays were examined and inhibitors of CXCR4 were used to determine the effect on cell migration. RESULTS: The cells isolated from subcutaneous adipose tissue show characteristics of stem cells. Gastric cancer cells express CXCR4 and Adipose-derived stem cells express SDF-1. Additionally, the tumor cells showed vigorous proliferative and migratory responses to ADSCs. AMD3100 can effectively reduce the migration of gastric cancer cells. CONCLUSIONS: Adipose-derived stem cells can promote gastric cancer cell growth, migration and invasion through SDF-1/CXCR4 axis. CXCR4 can be used in the treatment of gastric cancer as a potential therapeutic target.
Authors: Erhong Meng; Beverely Long; Paula Sullivan; Steve McClellan; Michael A Finan; Eddie Reed; Lalita Shevde; Rodney P Rocconi Journal: Clin Exp Metastasis Date: 2012-05-18 Impact factor: 5.150
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