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Literature DB >> 21431735

Visualization of mRNA localization in Xenopus oocytes.

Abstract

Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.

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Year: 2011 PMID： 21431735 PMCID： PMC3181151 DOI： 10.1007/978-1-61779-005-8_5

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

21 in total

1. Visualizing the distribution and transport of mRNAs in living cells.

Authors: Diana P Bratu; Byeong-Jik Cha; Musa M Mhlanga; Fred Russell Kramer; Sanjay Tyagi
Journal: Proc Natl Acad Sci U S A Date: 2003-10-28 Impact factor: 11.205

2. Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.

Authors: K L Mowry; D A Melton
Journal: Science Date: 1992-02-21 Impact factor: 47.728

3. The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.

Authors: J K Yisraeli; D A Melton
Journal: Nature Date: 1988-12-08 Impact factor: 49.962

4. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.

Authors: J N Dumont
Journal: J Morphol Date: 1972-02 Impact factor: 1.804

5. Growth of anuran oocytes in serum-supplemented medium.

Authors: R A Wallace; Z Misulovin; H S Wiley
Journal: Reprod Nutr Dev Date: 1980

6. Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.

Authors: Timothy J Messitt; James A Gagnon; Jill A Kreiling; Catherine A Pratt; Young J Yoon; Kimberly L Mowry
Journal: Dev Cell Date: 2008-09-04 Impact factor: 12.270

7. Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin.

Authors: Young J Yoon; Kimberly L Mowry
Journal: Development Date: 2004-05-26 Impact factor: 6.868

8. A critical role for Xdazl, a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus.

Authors: D W Houston; M L King
Journal: Development Date: 2000-02 Impact factor: 6.868

9. A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA.

Authors: J K Yisraeli; S Sokol; D A Melton
Journal: Development Date: 1990-02 Impact factor: 6.868

6. Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species.

Authors: Maike Claußen; Thomas Lingner; Claudia Pommerenke; Lennart Opitz; Gabriela Salinas; Tomas Pieler
Journal: Mol Biol Cell Date: 2015-09-02 Impact factor: 4.138

6 in total

Visualization of mRNA localization in Xenopus oocytes.

1. Visualizing the distribution and transport of mRNAs in living cells.

2. Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.

3. The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.

4. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.

5. Growth of anuran oocytes in serum-supplemented medium.

6. Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.

7. Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin.

8. A critical role for Xdazl, a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus.

9. A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA.

10. Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2.

1. Essential elements for translation: the germline factor Vasa functions broadly in somatic cells.

2. Methods for the analysis of early oogenesis in Zebrafish.

3. Using in vivo imaging to measure RNA mobility in Xenopus laevis oocytes.

4. Localisation of RNAs into the germ plasm of vitellogenic Xenopus oocytes.

5. Directional transport is mediated by a Dynein-dependent step in an RNA localization pathway.

6. Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species.